RT Journal Article SR Electronic(1) A1 Kumar, P. Anil A1 Aparna, P. A1 Srinivas, T. N. R. A1 Sasikala, Ch. A1 Ramana, Ch. V.YR 2008 T1 Rhodovulum kholense sp. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 7 SP 1723 OP 1726 DO https://doi.org/10.1099/ijs.0.65620-0 PB Microbiology Society, SN 1466-5034, AB A yellowish brown bacterium was isolated from photoheterotrophic enrichment cultures obtained from a mud sample collected from a mangrove forest located in Khola, India. Enrichment and isolation in medium containing 2 % NaCl (w/v) yielded strain JA297T, cells of which were ovoid and motile. Cells of strain JA297T contained vesicular internal membranes and bacteriochlorophyll a and carotenoids of the spheroidene series. Strain JA297T grew optimally at 30 °C and at pH 6.0–7.0. Photo-organoheterotrophy was the preferred mode of growth. Strain JA297T was also able to grow photolithoautotrophically and chemo-organotrophically but not by chemolithoautotrophy or by fermentation. Strain JA297T was able to utilize sulfide, sulfite, sulfate, thiosulfate, thioglycolate and cysteine as sulfur sources. Biotin, niacin and thiamine were required as growth factors. On the basis of 16S rRNA gene sequence analysis, strain JA297T was shown to belong to the class Alphaproteobacteria and was closely related to the type strains of Rhodovulum marinum (96.72 % similarity), Rhodovulum visakhapatnamense (96.42 %) and Rhodovulum sulfidophilum (96.32 %). The DNA G+C composition was 63 mol%. Based on 16S rRNA gene sequence analysis and morphological and physiological characteristics, strain JA297T could be distinguished from all recognized species of the genus Rhodovulum. This strain is therefore suggested to represent a novel species of the genus Rhodovulum, for which the name Rhodovulum kholense sp. nov. is proposed. The type strain is JA297T (=ATCC BAA-1544T=JCM 14888T=CCUG 55397T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65620-0