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Abstract

Strain HAL40b was isolated from the marine sponge sp. 1 collected at the Sula Ridge off the Norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. The isolate was a small rod with a polar flagellum. It was aerobic, Gram-negative and oxidase- and catalase-positive. Optimal growth was observed at 20–30 °C, pH 7–9 and in 3 % NaCl. Substrate utilization tests were positive for arabinose, Tween 40 and Tween 80. Enzyme tests were positive for alkaline phosphatase, esterase lipase (C8), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and -acetyl--glucosaminidase. The predominant cellular fatty acid was C 8, followed by C and C 7. Analysis by matrix-assisted laser desorption/ionization time-of-flight MS was used to characterize the strain, producing a characteristic low-molecular-mass protein pattern that could be used as a fingerprint for identification of members of this species. The DNA G+C content was 69.1 mol%. Phylogenetic analysis supported by 16S rRNA gene sequence comparison classified the strain as a member of the class . Strain HAL40b was only distantly related to other marine bacteria including and (type strain sequence similarity >90 %). Based on its phenotypic, physiological and phylogenetic characteristics, it is proposed that the strain should be placed into a new genus as a representative of a novel species, gen. nov., sp. nov.; the type strain of is HAL40b (=DSM 17750 =CCUG 54896).

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2008-03-01
2020-01-29
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vol. , part 3, pp. 585 - 590

MALDI-TOF mass spectrum of HAL40b in the mass range of 2000–10000 Da.

Whole-cell fatty acid composition of strain HAL40b .

[PDF file of Supplementary Fig. S1 and Supplementary Table S1](109 KB)



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