1887

Abstract

Six Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped isolates were obtained from fruit powder (=3), infant formula (=2) and an infant formula production environment (=1) and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with gene sequence analysis allocated the isolates to the family . The highest gene sequence similarities (91.2–95.8 %) were obtained with , , and and the phylogenetic branch formed by these species was supported by a high bootstrap value. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by their ability to utilize melibiose, sucrose, -arabitol, mucate and 1--methyl--galactopyranoside and their negative reactions for -sorbitol utilization and the Voges–Proskauer test. On the basis of the phylogenetic analyses, DNA–DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the isolates represent a novel species of the genus , sp. nov. The type strain is 601/05 (=LMG 24057=DSM 19144).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65427-0
2008-01-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/237.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65427-0&mimeType=html&fmt=ahah

References

  1. Anonymous ( 2006; ). ISO/TS 22964:2006 Milk and Milk Products – Detection of Enterobacter sakazakii, IDF/RM 210. Geneva, Switzerland: ISO.
  2. Drancourt, M., Bollet, C., Carta, A. & Rousselier, P. ( 2001; ). Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 51, 925–932.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Farmer, J. J., III, Boatwright, K. D. & Janda, J. M. ( 2007; ). Enterobacteriaceae: introduction and identification. In Manual of Clinical Microbiology, 9th edn, vol. 1, chapter 42, p. 649. Edited by J. H. Jorgensen. Washington, DC: American Society for Microbiology.
  5. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  6. Hoffmann, H., Stindl, S., Ludwig, W., Stumpf, A., Mehlen, A., Heesemann, J., Monget, D., Schleifer, K. H. & Roggenkamp, A. ( 2005; ). Reassignment of Enterobacter dissolvens to Enterobacter cloacae as E. cloacae subspecies dissolvens comb. nov. and emended description of Enterobacter asburiae and Enterobacter kobei. Syst Appl Microbiol 28, 196–205.[CrossRef]
    [Google Scholar]
  7. Inoue, K., Sugiyama, K., Kosako, Y., Sakazaki, R. & Yamai, S. ( 2000; ). Enterobacter cowanii sp. nov., a new species of the Enterobacteriaceae. Curr Microbiol 41, 417–421.[CrossRef]
    [Google Scholar]
  8. Iversen, C., Waddington, M., On, S. L. & Forsythe, S. ( 2004a; ). Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species. J Clin Microbiol 42, 5368–5370.[CrossRef]
    [Google Scholar]
  9. Iversen, C., Druggan, P. & Forsythe, S. J. ( 2004b; ). A selective differential medium for Enterobacter sakazakii, a preliminary study. Int J Food Microbiol 96, 133–139.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Ruppel, S. & Remus, R. ( 2005; ). Enterobacter radicincitans sp. nov., a plant growth promoting species of the family Enterobacteriaceae. Syst Appl Microbiol 28, 213–221.[CrossRef]
    [Google Scholar]
  11. Lehner, A. & Stephan, R. ( 2004; ). Microbiological, epidemiological and food safety aspects of Enterobacter sakazakii. J Food Prot 67, 2850–2857.
    [Google Scholar]
  12. Lehner, A., Tasara, T. & Stephan, R. ( 2004; ). 16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identification. BMC Microbiol 4, 43 [CrossRef]
    [Google Scholar]
  13. Lehner, A., Nitzsche, S., Breeuwer, P., Diep, B., Thelen, K. & Stephan, R. ( 2006; ). Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection. BMC Microbiol 6, 15 [CrossRef]
    [Google Scholar]
  14. Li, X., Zhang, D., Chen, F., Ma, J., Dong, Y. & Zhang, L. ( 2004; ). Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium. Int J Syst Evol Microbiol 54, 2131–2136.[CrossRef]
    [Google Scholar]
  15. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Mollet, C., Drancourt, M. & Raoult, D. ( 1997; ). rpoB sequence analysis as a novel basis for bacterial identification. Mol Microbiol 26, 1005–1011.[CrossRef]
    [Google Scholar]
  18. Niemann, S., Puehler, A., Tichy, H. V., Simon, R. & Selbitschka, W. ( 1997; ). Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. J Appl Microbiol 82, 477–484.[CrossRef]
    [Google Scholar]
  19. Richard, C. ( 1984; ). Genus VI. Enterobacter Hormaeche and Edwards 1960. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 465–469. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  20. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  21. Stephan, R., Van Trappen, S., Cleenwerck, I., Vancanneyt, M., De Vos, P. & Lehner, A. ( 2007; ). Enterobacter turicensis, sp. nov. and Enterobacter helveticus, sp. nov., isolated from fruit powder. Int J Syst Evol Microbiol 57, 820–826.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  23. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  24. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  25. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65427-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65427-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 237 - 241

Neighbour-joining tree of 16S rRNA gene sequences showing the estimated phylogenetic relationships between sp. nov. and related genera within the family . [ PDF] (394 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error