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Six Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped isolates were obtained from fruit powder (n=3), infant formula (n=2) and an infant formula production environment (n=1) and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated the isolates to the family Enterobacteriaceae. The highest rpoB gene sequence similarities (91.2–95.8 %) were obtained with Enterobacter helveticus, Enterobacter radicincitans, Enterobacter turicensis and Enterobacter sakazakii and the phylogenetic branch formed by these species was supported by a high bootstrap value. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by their ability to utilize melibiose, sucrose, d-arabitol, mucate and 1-O-methyl-α-galactopyranoside and their negative reactions for d-sorbitol utilization and the Voges–Proskauer test. On the basis of the phylogenetic analyses, DNA–DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the isolates represent a novel species of the genus Enterobacter, Enterobacter pulveris sp. nov. The type strain is 601/05T (=LMG 24057T=DSM 19144T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 1, pp. 237 - 241
Supplementary Fig. S1. Neighbour-joining tree of 16S rRNA gene sequences showing the estimated phylogenetic relationships between Enterobacter pulveris sp. nov. and related genera within the family Enterobacteriaceae . [ PDF] (394 KB)