%0 Journal Article %A Stephan, Roger %A Van Trappen, Stefanie %A Cleenwerck, Ilse %A Iversen, Carol %A Joosten, Han %A De Vos, Paul %A Lehner, Angelika %T Enterobacter pulveris sp. nov., isolated from fruit powder, infant formula and an infant formula production environment %D 2008 %J International Journal of Systematic and Evolutionary Microbiology, %V 58 %N 1 %P 237-241 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.65427-0 %K BPW, buffered peptone water %I Microbiology Society, %X Six Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped isolates were obtained from fruit powder (n=3), infant formula (n=2) and an infant formula production environment (n=1) and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated the isolates to the family Enterobacteriaceae. The highest rpoB gene sequence similarities (91.2–95.8 %) were obtained with Enterobacter helveticus, Enterobacter radicincitans, Enterobacter turicensis and Enterobacter sakazakii and the phylogenetic branch formed by these species was supported by a high bootstrap value. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by their ability to utilize melibiose, sucrose, d-arabitol, mucate and 1-O-methyl-α-galactopyranoside and their negative reactions for d-sorbitol utilization and the Voges–Proskauer test. On the basis of the phylogenetic analyses, DNA–DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the isolates represent a novel species of the genus Enterobacter, Enterobacter pulveris sp. nov. The type strain is 601/05T (=LMG 24057T=DSM 19144T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65427-0