A polyphasic taxonomic study was performed to compare 26 novel bacterial isolates obtained from (semi-)natural grassland soils and a heathland soil in the Netherlands with 16 strains that had previously been assigned to the genus . Genomic fingerprinting (BOX-PCR), whole-cell protein electrophoresis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry of intact cells and physiological characterization (Biolog) of the isolates confirmed the existence of different strain clusters (A–D) within the genus . Until now, only cluster C strains have been formally classified, as . In this study, DNA–DNA hybridizations were performed with a selection of strains representing the four clusters. The results showed that cluster B strains also belong to and that strains of clusters A and D represent two novel species within the genus . The latter novel species could be differentiated by means of phenotypic and genotypic characteristics and are classified as sp. nov. (cluster A; type strain Ter10 =LMG 23964 =CCUG 54727) and sp. nov. (cluster D; type strain Ter91 =LMG 23965 =CCUG 54728).


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vol. , part 2, pp. 414 - 419

DNA–DNA relatedness (%) among the seven tested strains. [PDF](16 KB)


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