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A Gram-positive, rod-shaped acid-fast bacterium was isolated from a patient with a post-traumatic chronic skin abscess associated with osteitis. Morphological analysis, 16S rRNA, hsp65, sodA and rpoB gene sequence analysis, cell-wall fatty acid and mycolic acid composition analyses and biochemical tests showed that the isolate, designated ABO-M06T, belonged to the genus Mycobacterium. Its phenotype was unique and genetic and phylogenetic findings suggest that strain ABO-M06T represents a novel species within the Mycobacterium fortuitum group. The name Mycobacterium setense sp. nov. is proposed for this novel species, with the type strain ABO-M06T (=CIP 109395T=DSM 45070T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 2, pp. 486–490
Supplementary Fig. S1. HPLC profile of M. setense sp. nov. ABO-M06 Tcompared with that of M. fortuitum CIP 104534 T, M. senegalense CIP 104941 Tand M. houstonense ATCC 49403 T.
Supplementary Fig. S2. Phylogenetic tree based on a partial hsp65 gene sequence, showing the relationship of M. setense ABO-M06 Twith the 14 most closely related Mycobacterium species.
Supplementary Fig. S3. Phylogenetic tree based on a partial sodA gene sequence, showing the relationship of M. setense ABO-M06 Twith the 14 most closely related Mycobacterium species.
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