A Gram-negative, aerobic bacterium, H1-M8, was isolated from seashore sand in Korea and then characterized using a polyphasic approach. Cells were short rods (0.7–1.0×1.5–2.0 μm) and were motile (each cell having at least one flagellum). Colonies were light-brown, non-pigmented, circular and convex with clear margins. Growth of the strain was observed at 5–35 °C, pH 6.0–9.0 and NaCl concentrations up to 8.4 % (w/v). Phylogenetic analysis of the 16S rRNA gene sequence revealed a clear affiliation between the novel strain and members of the genus . The sequence similarities between H1-M8 and type strains of the genus ranged from 97.0 to 97.8 %. However, DNA–DNA hybridizations between the isolate and type strains of other related species produced low values (21–38 %). The major isoprenoid quinone was Q-10 and the predominant cellular fatty acids were 18 : 17 (68.2 %) and 18 : 0 (10.5 %). The G+C content of the DNA was 63.6 mol%. On the basis of physiological, biochemical and chemotaxonomic traits and data from the comparative 16S rRNA sequence analysis, strain H1-M8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H1-M8 (=KACC 11609 =DSM 19073).


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Transmission electron micrograph of a cell of strain H1-M8 . Cells were placed on a carbon-coated copper grid and negatively stained with uranyl acetate. Bar, 500 nm.


Cellular fatty acid compositions (%) of strain H1-M8 and type strains of species. [PDF](35 KB)


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