1887

Abstract

A Gram-negative, coccobacillus-shaped, aerobic bacterium, designated strain UST040201-002, was isolated in February 2004 from seawater at the outlet of a sandfilter in Port Shelter, Hong Kong SAR, China. This strain possessed ubiquinone-8; its 16S rRNA gene sequence shared only 91 % similarity with the sequence from and 89–90 % similarity with sequences from , , and . 16S rRNA gene sequence analysis showed that the strain formed a distinct clade with . This subcluster formed a tight coherent group with members of the family and . Combined phylogenetic and physiological data suggest that strain UST040201-002 represents a novel genus and species within the order . The name gen. nov., sp. nov. is proposed; the type strain is UST040201-002 (=JCM 14605=NRRL B-41860).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65156-0
2007-11-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2665.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65156-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Baumann, P. & Baumann, L. ( 1981; ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. Schlegel. Berlin: Springer-Verlag.
  3. Beier, C. L., Horn, M., Michel, R., Schweikert, M., Gortz, H. D. & Wagner, M. ( 2002; ). The genus Caedibacter comprises endosymbionts of Paramecium spp. related to the Rickettsiales (Alphaproteobacteria) and to Francisella tularensis (Gammaproteobacteria). Appl Environ Microbiol 68, 6043–6050.[CrossRef]
    [Google Scholar]
  4. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  5. Collins, M. D. ( 1994; ). Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics, pp. 265–309. Edited by M. Goodfellow & A. G. O'Donnell. Chichester, UK: Wiley.
  6. Collins, C. H., Lyne, P. M. & Grange, J. M. ( 1989; ). Collins and Lyne's Microbiological Methods. London/Boston: Butterworths.
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  9. Hollis, D. G., Weaver, R. E., Steigerwalt, A. G., Wenger, J. D., Moss, C. W. & Brenner, D. J. ( 1989; ). Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease. J Clin Microbiol 27, 1601–1608.
    [Google Scholar]
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  12. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester, UK: Wiley.
  13. Larsson, P., Oyston, P. C., Chain, P., Chu, M. C., Duffield, M., Fuxelius, H. H., Garcia, E., Halltorp, G., Johansson, D. & other authors ( 2005; ). The complete genome sequence of Francisella tularensis, the causative agent of tularemia. Nat Genet 37, 153–159.[CrossRef]
    [Google Scholar]
  14. Lau, K. W., Ng, C. Y., Ren, J., Lau, S. C., Qian, P. Y., Wong, P. K., Lau, T. C. & Wu, M. ( 2005; ). Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’. Int J Syst Evol Microbiol 55, 1051–1057.[CrossRef]
    [Google Scholar]
  15. Lewin, R. A. & Lounsbery, D. M. ( 1969; ). Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58, 145–170.[CrossRef]
    [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Olsufjev, N. G., Emelyanova, O. S. & Dunaeva, T. N. ( 1959; ). Comparative study of strains of B. tularense in the Old and New World and their taxonomy. J Hyg Epidemiol Microbiol Immunol 3, 138–149.
    [Google Scholar]
  18. Olsufjev, N. G. & Meshcheryakova, I. S. ( 1983; ). Subspecific taxonomy of Francisella tularensis McCoy and Chapin 1912. Int J Syst Bacteriol 33, 872–874.[CrossRef]
    [Google Scholar]
  19. Reichenbach, H. ( 1989; ). Family 1. Cytophagaceae Stanier 1940, 630AL emend. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2013–2082. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Sjöstedt, A. B. ( 2005; ). Genus I. Francisella Dorofe'ev 1947, 176AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 200–210. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  22. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65156-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65156-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2665 - 2669

Photomicrographs of strain UST040201-002 . [PDF](113 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error