1887

Abstract

Two Gram-negative, cold-adapted, aerobic bacteria, designated strains M8 and M6, were isolated from soil collected from the South Shetland Islands. The organisms were rod-shaped, catalase- and oxidase-positive and motile by means of polar flagella. These two psychrotolerant strains grew between −4 and 30 °C. 16S rRNA gene sequence analysis placed strains M8 and M6 within the genus . DNA–DNA hybridization experiments between the Antarctic isolate M8 and type strains of phylogenetically related species, namely and , revealed levels of relatedness of 33 and 37 %, respectively. Strain M6 showed 99 % DNA similarity to strain M8. Several phenotypic characteristics, together with data on cellular fatty acid composition, served to differentiate strains M8 and M6 from related pseudomonads. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains M8 and M6 belong to the same genospecies, representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M8 (=LMG 24016=CECT 7231).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65141-0
2007-11-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2609.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65141-0&mimeType=html&fmt=ahah

References

  1. Antranikian, G., Vorgias, C. E. & Bertoldo, C. ( 2005; ). Extreme environments as a source for microorganisms and novel biocatalysts. Adv Biochem Eng Biotechnol 96, 219–262.
    [Google Scholar]
  2. Anzai, Y., Kim, H., Park, J. Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  3. Bozal, N., Montes, M. J., Tudela, E., Jiménez, F. & Guinea, J. ( 2002; ). Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52, 195–205.
    [Google Scholar]
  4. Bruni, V., Gugliandolo, C., Maugeri, T. & Allegra, A. ( 1999; ). Psychrotrophic bacteria from a coastal station in the Ross Sea (Terra Nova Bay, Antarctica). New Microbiol 22, 357–363.
    [Google Scholar]
  5. Cleenwerck, I., Vandemeulebroecke, K., Janssens, D. & Swings, J. ( 2002; ). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52, 1551–1588.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1993; ). Manual for the Identification of Medical Bacteria, 3rd edn. Edited by G. I. Barrow & R. K. A. Feltham. Cambridge: Cambridge University Press.
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  8. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  9. Kersters, K., Ludwig, W., Vancanneyt, M., De Vos, P., Gillis, M. & Schleifer, K. H. ( 1996; ). Recent changes in the classification of the pseudomonads: an overview. Syst Appl Microbiol 19, 465–477.[CrossRef]
    [Google Scholar]
  10. King, E. O., Ward, M. K. & Rainey, D. E. ( 1954; ). Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44, 301–307.
    [Google Scholar]
  11. Kriss, A. E., Mitskevich, I. N., Rozanova, E. P. & Osnitskaia, L. K. ( 1976; ). Microbiological studies of the Wanda Lake (Antarctica). Microbiology (English translation of Mikrobiologiya) 45, 1075–1081 (in Russian).
    [Google Scholar]
  12. Ma, Y., Wang, L. & Shao, Z. ( 2006; ). Pseudomonas, the dominant polycyclic aromatic hydrocarbon-degrading bacteria isolated from Antarctic soils and the role of large plasmids in horizontal gene transfer. Environ Microbiol 8, 455–465.[CrossRef]
    [Google Scholar]
  13. Maugeri, T. L., Gugliandolo, C. & Bruni, V. ( 1996; ). Heterotrophic bacteria in the Ross Sea (Terra Nova Bay, Antarctica). New Microbiol 19, 67–76.
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Niemann, S., Puehler, A., Tichi, H. V., Simon, R. & Selbitschka, W. ( 1997; ). Evaluation of the resolving power of the three different fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. J Appl Microbiol 82, 477–484.[CrossRef]
    [Google Scholar]
  16. Reddy, G. S. N., Matsumoto, G. I., Schumann, P., Stackebrandt, E. & Shivaji, S. ( 2004; ). Psychrophilic pseudomonads from Antarctica: Pseudomonas antarctica sp. nov. and Pseudomonas proteolytica sp. nov. Int J Syst Evol Microbiol 54, 713–719.[CrossRef]
    [Google Scholar]
  17. Shivaji, S., Rao, N. S., Saisree, L., Sheth, V., Reddy, G. S. N. & Bhargava, P. M. ( 1989; ). Isolation and identification of Pseudomonas spp. from Schirmacher Oasis, Antarctica. Appl Environ Microbiol 55, 767–770.
    [Google Scholar]
  18. Vanparys, B., Heylen, K., Lebbe, L. & De Vos, P. ( 2006; ). Pseudomonas peli sp. nov. and Pseudomonas borbori sp.nov., isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 56, 1875–1881.[CrossRef]
    [Google Scholar]
  19. Wakabayashi, H. & Egusa, S. ( 1972; ). Characteristics of a Pseudomonas sp. from an epizootic of pound-cultured eels (Anguilla japonica). Bull Jpn Soc Sci Fish 38, 577–587.[CrossRef]
    [Google Scholar]
  20. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  21. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65141-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65141-0
Loading

Data & Media loading...

Supplements

Electron micrograph of a negatively stained cell of strain M8 , showing a polar flagellum. Bar, 1 μm.

IMAGE

[ Single PDF file] (64 KB)

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error