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Two novel Gram-negative bacteria, designated strains DR41_21T and DR41_18T, were isolated from coastal, surface waters of the north-western Mediterranean Sea. The cells were motile, pleomorphic rods, 2.9 μm long and 0.9 μm wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 60 mol%. Phylogenetic analysis of 16S rRNA gene sequences positioned the isolates in the class Alphaproteobacteria within the family Rhodospirillaceae. The 16S rRNA gene sequence similarity of the two strains was 98.8 % but DNA–DNA hybridization indicated only 55 % relatedness. Strain DR41_21T was able to denitrify and possessed nirK and nosZ genes, unlike strain DR41_18T, which possessed only nirK. These isolates represent two novel species of a new genus, Nisaea gen. nov., for which the names Nisaea denitrificans sp. nov. and Nisaea nitritireducens sp. nov. are proposed. The type strain of Nisaea denitrificans is DR41_21T (=DSM 18348T=CIP 109265T=OOB 129T) and the type strain of Nisaea nitritireducens is DR41_18T (=DSM 19540T=CIP 109601T=OOB 128T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 10, pp. 2336 - 2341
Supplementary Fig. S1. Electron micrograph of a negatively stained cell of strain DR41_21 Tharvested during the exponential phase.
Supplementary Fig. S2. Effects of temperature, pH and NaCl concentration on the growth of strain DR41_21 T.
Supplementary Fig. S3. Fatty acid composition analysis for strains DR41_18 Tand DR41_21 T.
Supplementary Fig. S4. Analysis of polar lipids for strains DR41_18 Tand DR41_21 T.
Supplementary Fig. S5. Phylogenetic analysis of nirK sequences of strains DR41_21 Tand DR41_18 T.
Supplementary Fig. S6. Phylogenetic analysis of the nosZ sequence of strain DR41_21 T.
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