RT Journal Article SR Electronic(1) A1 Urios, Laurent A1 Michotey, Valérie A1 Intertaglia, Laurent A1 Lesongeur, Françoise A1 Lebaron, PhilippeYR 2008 T1 Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 10 SP 2336 OP 2341 DO https://doi.org/10.1099/ijs.0.64592-0 PB Microbiology Society, SN 1466-5034, AB Two novel Gram-negative bacteria, designated strains DR41_21T and DR41_18T, were isolated from coastal, surface waters of the north-western Mediterranean Sea. The cells were motile, pleomorphic rods, 2.9 μm long and 0.9 μm wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 60 mol%. Phylogenetic analysis of 16S rRNA gene sequences positioned the isolates in the class Alphaproteobacteria within the family Rhodospirillaceae. The 16S rRNA gene sequence similarity of the two strains was 98.8 % but DNA–DNA hybridization indicated only 55 % relatedness. Strain DR41_21T was able to denitrify and possessed nirK and nosZ genes, unlike strain DR41_18T, which possessed only nirK. These isolates represent two novel species of a new genus, Nisaea gen. nov., for which the names Nisaea denitrificans sp. nov. and Nisaea nitritireducens sp. nov. are proposed. The type strain of Nisaea denitrificans is DR41_21T (=DSM 18348T=CIP 109265T=OOB 129T) and the type strain of Nisaea nitritireducens is DR41_18T (=DSM 19540T=CIP 109601T=OOB 128T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64592-0