1887

Abstract

A Gram-positive, non-motile, endospore-forming bacterial strain, designated Gsoil 420, was isolated from soil of a ginseng field in Pocheon Province, South Korea, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. The novel isolate consisted of strictly aerobic, rod-shaped cells and was able to grow in medium supplemented with up to 12 % NaCl at 25 °C and pH 6.5–7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 420 fell within the radiation of the cluster comprising species and formed a coherent cluster with (16S rRNA gene sequence similarity, 98.6 %), (98.6 %), (98.3 %), (98.0 %), (98.0 %), (97.9 %), (97.6 %), (97.4 %) and (97.0 %). The levels of 16S rRNA gene sequence similarity with respect to other species with validly published names were less than 96.8 %. Strain Gsoil 420 had a genomic DNA G+C content of 44.9 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were anteiso-C (33.9 %), iso-C (24.5 %) and iso-C (19.9 %). These chemotaxonomic results supported the affiliation of strain Gsoil 420 to the genus However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain Gsoil 420 from recognized species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 420 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gsoil 420 (=KCTC 13943=DSM 18135).

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2007-11-01
2020-01-29
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vol. , part 11, pp. 2532 - 2537

Expanded neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, showing the position of strain Gsoil 420 with respect to species of the genus

Levels of DNA–DNA relatedness [Single PDF file](31 KB)



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