Two anaerobic, non-spore-forming, bacteria (YIT 11850 and YIT 11860) that stained Gram-negative, were isolated from human faeces. Cells of strain YIT 11850 were coccobacilli, asaccharolytic and largely unreactive, with only traces of lactate and propionate as metabolic end products; however, strain YIT 11850 was able to decarboxylate succinate to propionate. The DNA G+C content of strain YIT 11850 was 51.9 mol%. Following 16S rRNA gene sequence analysis, this strain was found to be most closely related to , with 95.1 % sequence similarity between the two taxa. Biochemical data supported the affiliation of strain YIT 11850 to the genus . Strain YIT 11850 therefore represents a novel species for which the name sp. nov. is proposed; the type strain is YIT 11850 (=DSM 21274=JCM 15077). Cells of the other isolate, strain YIT 11860, were non-motile, rod-shaped, positive for aesculin hydrolysis, negative for indole production, produced succinic and acetic acids as end products of glucose metabolism and possessed a DNA G+C content of 45.5 mol%. On the basis of 16S rRNA gene sequence similarity values, this strain was shown to belong to the family ‘’ related to (96.0 %); similarity values with species within the family ‘’ with validly published names were less than 86 %. Biochemical data supported the affiliation of strain YIT 11860 to the genus . Strain YIT 11860 therefore represents a novel species for which the name sp. nov. is proposed; the type strain is YIT 11860 (=DSM 21032=JCM 15079).

Keyword(s): GI, gastrointestinal

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vol. , part 12, pp. 2716 - 2720

Similarity values (%) for the 16S rRNA gene sequences of sp. nov. and other members of the genus . [ PDF] 29 KB


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