1887

Abstract

Two anaerobic, non-spore-forming, bacteria (YIT 11850 and YIT 11860) that stained Gram-negative, were isolated from human faeces. Cells of strain YIT 11850 were coccobacilli, asaccharolytic and largely unreactive, with only traces of lactate and propionate as metabolic end products; however, strain YIT 11850 was able to decarboxylate succinate to propionate. The DNA G+C content of strain YIT 11850 was 51.9 mol%. Following 16S rRNA gene sequence analysis, this strain was found to be most closely related to , with 95.1 % sequence similarity between the two taxa. Biochemical data supported the affiliation of strain YIT 11850 to the genus . Strain YIT 11850 therefore represents a novel species for which the name sp. nov. is proposed; the type strain is YIT 11850 (=DSM 21274=JCM 15077). Cells of the other isolate, strain YIT 11860, were non-motile, rod-shaped, positive for aesculin hydrolysis, negative for indole production, produced succinic and acetic acids as end products of glucose metabolism and possessed a DNA G+C content of 45.5 mol%. On the basis of 16S rRNA gene sequence similarity values, this strain was shown to belong to the family ‘’ related to (96.0 %); similarity values with species within the family ‘’ with validly published names were less than 86 %. Biochemical data supported the affiliation of strain YIT 11860 to the genus . Strain YIT 11860 therefore represents a novel species for which the name sp. nov. is proposed; the type strain is YIT 11860 (=DSM 21032=JCM 15079).

Keyword(s): GI, gastrointestinal
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.2008/000810-0
2008-12-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2716.html?itemId=/content/journal/ijsem/10.1099/ijs.0.2008/000810-0&mimeType=html&fmt=ahah

References

  1. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M. ( 1923; ). In Bergey's Manual of Determinative Bacteriology, pp. 271–272. Baltimore: Williams & Wilkins.
  2. Chonan, O., Matsumoto, K. & Watanuki, M. ( 1995; ). Effect of galactooligosaccharides on calcium absorption and preventing bone loss in ovariectomized rats. Biosci Biotechnol Biochem 59, 236–239.[CrossRef]
    [Google Scholar]
  3. Downes, J., Munson, M. & Wade, W. G. ( 2003; ). Dialister invisus sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 53, 1937–1940.[CrossRef]
    [Google Scholar]
  4. Eckburg, P. B., Bik, E. M., Bernstein, C. N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S. R., Nelson, K. E. & Relman, D. A. ( 2005; ). Diversity of the human intestinal microbial flora. Science 308, 1635–1638.[CrossRef]
    [Google Scholar]
  5. Ezaki, T., Saidi, S. M., Liu, S. L., Hashimoto, Y., Yamamoto, H. & Yabuuchi, E. ( 1990; ). Rapid procedure to determine the DNA base composition from small amounts of gram-positive bacteria. FEMS Microbiol Lett 55, 127–130.
    [Google Scholar]
  6. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Gill, S. R., Pop, M., Deboy, R. T., Eckburg, P. B., Turnbaugh, P. J., Samuel, B. S., Gordon, J. I., Relman, D. A., Fraser-Liggett, C. M. & Nelson, K. E. ( 2006; ). Metagenomic analysis of the human distal gut microbiome. Science 312, 1355–1359.[CrossRef]
    [Google Scholar]
  8. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  9. Jumas-Bilak, E., Jean-Pierre, H., Carlier, J.-P., Teyssier, C., Bernard, K., Gay, B., Campos, J., Morio, F. & Marchandin, H. ( 2005; ). Dialister micraerophilus sp. nov. and Dialister propionicifaciens sp. nov., isolated from human clinical samples. Int J Syst Evol Microbiol 55, 2471–2478.[CrossRef]
    [Google Scholar]
  10. Ley, R. E., Turnbaugh, P. J., Klein, S. & Gordon, J. I. ( 2006; ). Microbial ecology: human gut microbes associated with obesity. Nature 444, 1022–1023.[CrossRef]
    [Google Scholar]
  11. Lipman, D. J. & Pearson, W. R. ( 1985; ). Rapid and sensitive protein similarity searches. Science 227, 1435–1441.[CrossRef]
    [Google Scholar]
  12. Mai, V., Greenwald, B., Morris, J. G., Jr, Raufman, J. P. & Stine, O. C. ( 2006; ). Effect of bowel preparation and colonoscopy on post-procedure intestinal microbiota composition. Gut 55, 1822–1823.[CrossRef]
    [Google Scholar]
  13. Moore, L. V. H. & Moore, W. E. C. ( 1994; ). Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., anaerobic gram-negative bacilli from the human gingival crevice. Int J Syst Bacteriol 44, 187–192.[CrossRef]
    [Google Scholar]
  14. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  15. Rajilić-Stojanović, M., Smidt, H. & de Vos, W. M. ( 2007; ). Diversity of the human gastrointestinal tract microbiota revisited. Environ Microbiol 9, 2125–2136.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Sakamoto, M., Lan, P. T. N. & Benno, Y. ( 2007; ). Barnesiella viscericola gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from chicken caecum. Int J Syst Evol Microbiol 57, 342–346.[CrossRef]
    [Google Scholar]
  18. Sakon, H., Nagai, F., Morotomi, M. & Tanaka, R. ( 2008; ). Sutterella parvirubra sp. nov. and Megamonas funiformis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 58, 970–975.[CrossRef]
    [Google Scholar]
  19. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  21. Willems, A. & Collins, M. D. ( 1995; ). Phylogenetic placement of Dialister pneumosintes (formerly Bacteroides pneumosintes) within the Sporomusa subbranch of the Clostridium subphylum of the gram-positive bacteria. Int J Syst Bacteriol 45, 403–405.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.2008/000810-0
Loading
/content/journal/ijsem/10.1099/ijs.0.2008/000810-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2716 - 2720

Similarity values (%) for the 16S rRNA gene sequences of sp. nov. and other members of the genus . [ PDF] 29 KB



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error