RT Journal Article SR Electronic(1) A1 Morotomi, Masami A1 Nagai, Fumiko A1 Sakon, Hiroshi A1 Tanaka, RyuichiroYR 2008 T1 Dialister succinatiphilus sp. nov. and Barnesiella intestinihominis sp. nov., isolated from human faeces JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 12 SP 2716 OP 2720 DO https://doi.org/10.1099/ijs.0.2008/000810-0 PB Microbiology Society, SN 1466-5034, AB Two anaerobic, non-spore-forming, bacteria (YIT 11850T and YIT 11860T) that stained Gram-negative, were isolated from human faeces. Cells of strain YIT 11850T were coccobacilli, asaccharolytic and largely unreactive, with only traces of lactate and propionate as metabolic end products; however, strain YIT 11850T was able to decarboxylate succinate to propionate. The DNA G+C content of strain YIT 11850T was 51.9 mol%. Following 16S rRNA gene sequence analysis, this strain was found to be most closely related to Dialister propionicifaciens, with 95.1 % sequence similarity between the two taxa. Biochemical data supported the affiliation of strain YIT 11850T to the genus Dialister. Strain YIT 11850T therefore represents a novel species for which the name Dialister succinatiphilus sp. nov. is proposed; the type strain is YIT 11850T (=DSM 21274T=JCM 15077T). Cells of the other isolate, strain YIT 11860T, were non-motile, rod-shaped, positive for aesculin hydrolysis, negative for indole production, produced succinic and acetic acids as end products of glucose metabolism and possessed a DNA G+C content of 45.5 mol%. On the basis of 16S rRNA gene sequence similarity values, this strain was shown to belong to the family ‘Porphyromonadaceae’ related to Barnesiella viscericola (96.0 %); similarity values with species within the family ‘Porphyromonadaceae’ with validly published names were less than 86 %. Biochemical data supported the affiliation of strain YIT 11860T to the genus Barnesiella. Strain YIT 11860T therefore represents a novel species for which the name Barnesiella intestinihominis sp. nov. is proposed; the type strain is YIT 11860T (=DSM 21032T=JCM 15079T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.2008/000810-0