1887

Abstract

A Gram-stain-negative, non-spore-forming, non-motile, ovoid, aerobic bacterial strain, designated BUT-3, was isolated from activated sludge from the wastewater treatment facility of a herbicide-manufacturing plant in Kunshan city, Jiangsu province, PR China. Strain BUT-3 grew between 15 and 40 °C, with optimum growth at 30 °C. The pH range for growth was between 5.0 and 10.0 (optimum pH 7.0). The range of NaCl concentrations for growth of strain BUT-3 was 0–7.0 % (w/v), with an optimum of 1.5–3.0 % (w/v). A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain BUT-3 clustered closely with 120-1 (98.32 % similarity), with a bootstrap confidence level of 100 %. The major fatty acids (>5 % of total fatty acids) were C cyclo ω8, Cω7, C, anteiso-C and iso-C. Strain BUT-3 contained ubiquinone Q-10 as the predominant respiratory quinone. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three unidentified aminolipids (AL1–3), two unknown phospholipids (PL1, 5), four unidentified glycolipids (GL1–4) and two unknown lipids (L1, 2). The G+C content of the genomic DNA was 67.7 mol%. The DNA–DNA relatedness between BUT-3 and 120-1 was 44.1±0.6 %. Based on the polyphasic taxonomic data, strain BUT-3 should be classified as a representative of a novel species of the genus , for which the name Rhodoligotrophos sp. nov. is proposed. The type strain is BUT-3 ( = CCTCC AB2013083 = KACC 17220).

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2014-09-01
2019-11-17
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