RT Journal Article SR Electronic(1) A1 Deng, Shi-Kai A1 Chen, Guo-Qiang A1 Chen, Qing A1 Cai, Shu A1 Yao, Li A1 He, Jian A1 Li, Shun-PengYR 2014 T1 Rhodoligotrophosjinshengii sp. nov., isolated from activated sludge JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_9 SP 3325 OP 3330 DO https://doi.org/10.1099/ijs.0.066050-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, non-spore-forming, non-motile, ovoid, aerobic bacterial strain, designated BUT-3T, was isolated from activated sludge from the wastewater treatment facility of a herbicide-manufacturing plant in Kunshan city, Jiangsu province, PR China. Strain BUT-3T grew between 15 and 40 °C, with optimum growth at 30 °C. The pH range for growth was between 5.0 and 10.0 (optimum pH 7.0). The range of NaCl concentrations for growth of strain BUT-3T was 0–7.0 % (w/v), with an optimum of 1.5–3.0 % (w/v). A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain BUT-3T clustered closely with Rhodoligotrophos appendicifer 120-1T (98.32 % similarity), with a bootstrap confidence level of 100 %. The major fatty acids (>5 % of total fatty acids) were C19 : 0 cyclo ω8c, C18 : 1ω7c, C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. Strain BUT-3T contained ubiquinone Q-10 as the predominant respiratory quinone. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three unidentified aminolipids (AL1–3), two unknown phospholipids (PL1, 5), four unidentified glycolipids (GL1–4) and two unknown lipids (L1, 2). The G+C content of the genomic DNA was 67.7 mol%. The DNA–DNA relatedness between BUT-3T and R. appendicifer 120-1T was 44.1±0.6 %. Based on the polyphasic taxonomic data, strain BUT-3T should be classified as a representative of a novel species of the genus Rhodoligotrophos , for which the name Rhodoligotrophos jinshengii sp. nov. is proposed. The type strain is BUT-3T ( = CCTCC AB2013083T = KACC 17220T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.066050-0