1887

Abstract

A Gram-stain-negative bacterial strain, 10-17, was isolated from traditional sourdough in Heilongjiang Province, China. The bacterium was characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, RNA polymerase β subunit () gene sequence analysis, DNA gyrase () gene sequence analysis, initiation translation factor 2 () gene sequence analysis, ATP synthase β subunit () gene sequence analysis, fatty acid methyl ester analysis, determination of DNA G+C content, DNA–DNA hybridization and an analysis of phenotypic features. Strain 10-17 was phylogenetically related to CIP 103441, LMG 2693, JCM 6051, LMG 25706, EN-119 and LMG 2803, having 99.5 %, 99.3 %, 98.7 %, 98.5 %, 98.4 % and 98.4 % 16S rRNA gene sequence similarity, respectively. On the basis of polyphasic characterization data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is 10-17 ( = LMG 27195 = NCIMB 14836 = CCUG 62994). Zhu 2013 was reclassified as comb. nov. on the basis of 16S rRNA, , , and gene sequence analysis and the type strain is strain SP1( = CGMCC 1.12102 = LMG 26783).

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2014-08-01
2019-10-24
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