1887

Abstract

Two strains of lactic acid bacteria, Nu27 and Nu29, were isolated from Nukadoko, rice bran paste for Japanese pickles. The isolates were Gram-stain-positive, rod-shaped, catalase-negative, non-motile and facultatively anaerobic lactic acid bacteria. The isolates showed identical 16S rRNA gene sequences. The closest relatives to strain Nu27 based on 16S rRNA gene sequence similarities were KU-3 (98.9 % 16S rRNA gene sequence similarity), iz4b (96.3 %) and CECT 5290 (97.2 %). DNA–DNA relatedness values revealed genotype separation of the two isolates from the above three species. Based on the physiological, biochemical and genotypic characteristics provided, the isolates represent a novel species of the genus , for which name is proposed. The type strain is Nu 27 ( = JCM 18764 = NRIC 0900 = DSM 27174).

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2014-08-01
2019-10-18
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References

  1. Chenoll E., Macián M. C., Aznar R.. ( 2006;). Lactobacillus tucceti sp. nov., a new lactic acid bacterium isolated from sausage. . Syst Appl Microbiol 29:, 389–395. [CrossRef][PubMed]
    [Google Scholar]
  2. Darriba D., Taboada G. L., Doallo R., Posada D.. ( 2012;). jModelTest 2: more models, new heuristics and parallel computing. . Nat Methods 9:, 772. [CrossRef][PubMed]
    [Google Scholar]
  3. Endo A., Okada S.. ( 2006;). Oenococcus kitaharae sp. nov., a non-acidophilic and non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue. . Int J Syst Evol Microbiol 56:, 2345–2348. [CrossRef][PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Guindon S., Gascuel O.. ( 2003;). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef][PubMed]
    [Google Scholar]
  7. Irisawa T., Okada S.. ( 2009;). Lactobacillus sucicola sp. nov., a motile lactic acid bacterium isolated from oak tree (Quercus sp.) sap. . Int J Syst Evol Microbiol 59:, 2662–2665. [CrossRef][PubMed]
    [Google Scholar]
  8. Kashiwagi T., Suzuki T., Kamakura T.. ( 2009;). Lactobacillus nodensis sp. nov., isolated from rice bran. . Int J Syst Evol Microbiol 59:, 83–86. [CrossRef][PubMed]
    [Google Scholar]
  9. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  10. Kitahara M., Takamine F., Imamura T., Benno Y.. ( 2001;). Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7α-dehydroxylating activity. . Int J Syst Evol Microbiol 51:, 39–44.[PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  12. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). Clustal W and Clustal X version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  13. Manome A., Okada S., Uchimura T., Komagata K.. ( 1998;). The ratio of l-form to d-form of lactic acid as a criteria for the identification of lactic acid bacteria. . J Gen Appl Microbiol 44:, 371–374. [CrossRef][PubMed]
    [Google Scholar]
  14. Nakayama J., Hoshiko H., Fukuda M., Tanaka H., Sakamoto N., Tanaka S., Ohue K., Sakai K., Sonomoto K.. ( 2007;). Molecular monitoring of bacterial community structure in long-aged nukadoko: pickling bed of fermented rice bran dominated by slow-growing lactobacilli. . J Biosci Bioeng 104:, 481–489. [CrossRef][PubMed]
    [Google Scholar]
  15. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J.. ( 2005;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151:, 2141–2150. [CrossRef][PubMed]
    [Google Scholar]
  16. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J.. ( 2007;). Identification of lactobacilli by pheS and rpoA gene sequence analyses. . Int J Syst Evol Microbiol 57:, 2777–2789. [CrossRef][PubMed]
    [Google Scholar]
  17. Okada S., Hosoi Y., Takahashi M., Kozaki M.. ( 1991;). A rapid method for the determination of the fermentation types, homo- and hetero-fermentation, of lactic acid bacteria. . Bull JFCC 7:, 6–10. (in Japanese).
    [Google Scholar]
  18. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Sakamoto M., Ohkuma M.. ( 2013;). Bacteroides reticulotermitis sp. nov., isolated from the gut of a subterranean termite (Reticulitermes speratus). . Int J Syst Evol Microbiol 63:, 691–695. [CrossRef][PubMed]
    [Google Scholar]
  20. Sakamoto N., Tanaka S., Sonomoto K., Nakayama J.. ( 2011;). 16S rRNA pyrosequencing-based investigation of the bacterial community in nukadoko, a pickling bed of fermented rice bran. . Int J Food Microbiol 144:, 352–359. [CrossRef][PubMed]
    [Google Scholar]
  21. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  22. Tohno M., Kitahara M., Irisawa T., Inoue H., Uegaki R., Ohkuma M., Tajima K.. ( 2013;). Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica). . Int J Syst Evol Microbiol 63:, 2957–2962. [CrossRef][PubMed]
    [Google Scholar]
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