1887

Abstract

Two strains of lactic acid bacteria, Nu27 and Nu29, were isolated from Nukadoko, rice bran paste for Japanese pickles. The isolates were Gram-stain-positive, rod-shaped, catalase-negative, non-motile and facultatively anaerobic lactic acid bacteria. The isolates showed identical 16S rRNA gene sequences. The closest relatives to strain Nu27 based on 16S rRNA gene sequence similarities were KU-3 (98.9 % 16S rRNA gene sequence similarity), iz4b (96.3 %) and CECT 5290 (97.2 %). DNA–DNA relatedness values revealed genotype separation of the two isolates from the above three species. Based on the physiological, biochemical and genotypic characteristics provided, the isolates represent a novel species of the genus , for which name is proposed. The type strain is Nu 27 ( = JCM 18764 = NRIC 0900 = DSM 27174).

Funding
This study was supported by the:
  • Japan Science and Technology Agency (JST)/Japan International Cooperation Agency (JICA)
  • Science and Technology Research Partnership for Sustainable Development (SATREPS)
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2014-08-01
2024-11-03
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References

  1. Chenoll E., Macián M. C., Aznar R. ( 2006 ). Lactobacillus tucceti sp. nov., a new lactic acid bacterium isolated from sausage. . Syst Appl Microbiol 29, 389395. [View Article] [PubMed]
    [Google Scholar]
  2. Darriba D., Taboada G. L., Doallo R., Posada D. ( 2012 ). jModelTest 2: more models, new heuristics and parallel computing. . Nat Methods 9, 772. [View Article] [PubMed]
    [Google Scholar]
  3. Endo A., Okada S. ( 2006 ). Oenococcus kitaharae sp. nov., a non-acidophilic and non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue. . Int J Syst Evol Microbiol 56, 23452348. [View Article] [PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  6. Guindon S., Gascuel O. ( 2003 ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52, 696704. [View Article] [PubMed]
    [Google Scholar]
  7. Irisawa T., Okada S. ( 2009 ). Lactobacillus sucicola sp. nov., a motile lactic acid bacterium isolated from oak tree (Quercus sp.) sap. . Int J Syst Evol Microbiol 59, 26622665. [View Article] [PubMed]
    [Google Scholar]
  8. Kashiwagi T., Suzuki T., Kamakura T. ( 2009 ). Lactobacillus nodensis sp. nov., isolated from rice bran. . Int J Syst Evol Microbiol 59, 8386. [View Article] [PubMed]
    [Google Scholar]
  9. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  10. Kitahara M., Takamine F., Imamura T., Benno Y. ( 2001 ). Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7α-dehydroxylating activity. . Int J Syst Evol Microbiol 51, 3944.[PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  12. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). Clustal W and Clustal X version 2.0. . Bioinformatics 23, 29472948. [View Article] [PubMed]
    [Google Scholar]
  13. Manome A., Okada S., Uchimura T., Komagata K. ( 1998 ). The ratio of l-form to d-form of lactic acid as a criteria for the identification of lactic acid bacteria. . J Gen Appl Microbiol 44, 371374. [View Article] [PubMed]
    [Google Scholar]
  14. Nakayama J., Hoshiko H., Fukuda M., Tanaka H., Sakamoto N., Tanaka S., Ohue K., Sakai K., Sonomoto K. ( 2007 ). Molecular monitoring of bacterial community structure in long-aged nukadoko: pickling bed of fermented rice bran dominated by slow-growing lactobacilli. . J Biosci Bioeng 104, 481489. [View Article] [PubMed]
    [Google Scholar]
  15. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. ( 2005 ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151, 21412150. [View Article] [PubMed]
    [Google Scholar]
  16. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J. ( 2007 ). Identification of lactobacilli by pheS and rpoA gene sequence analyses. . Int J Syst Evol Microbiol 57, 27772789. [View Article] [PubMed]
    [Google Scholar]
  17. Okada S., Hosoi Y., Takahashi M., Kozaki M. ( 1991 ). A rapid method for the determination of the fermentation types, homo- and hetero-fermentation, of lactic acid bacteria. . Bull JFCC 7, 610. (in Japanese).
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Sakamoto M., Ohkuma M. ( 2013 ). Bacteroides reticulotermitis sp. nov., isolated from the gut of a subterranean termite (Reticulitermes speratus). . Int J Syst Evol Microbiol 63, 691695. [View Article] [PubMed]
    [Google Scholar]
  20. Sakamoto N., Tanaka S., Sonomoto K., Nakayama J. ( 2011 ). 16S rRNA pyrosequencing-based investigation of the bacterial community in nukadoko, a pickling bed of fermented rice bran. . Int J Food Microbiol 144, 352359. [View Article] [PubMed]
    [Google Scholar]
  21. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  22. Tohno M., Kitahara M., Irisawa T., Inoue H., Uegaki R., Ohkuma M., Tajima K. ( 2013 ). Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica). . Int J Syst Evol Microbiol 63, 29572962. [View Article] [PubMed]
    [Google Scholar]
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