1887

Abstract

A novel actinobacterial strain, designated MUSC 201, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201 represented a novel lineage within the class . Strain MUSC 201 formed a distinct clade in the family and was most closely related to the members of the genera (16S rRNA gene sequence similarity, 91.9–95.1 %), (92.7–94.6 %), (92.5–93.1 %) and (91.5–92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained -diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3γ. The peptidoglycan cell wall contained -diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5 : 0.9 : 1.0 : 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were Cω9 (30.8 %), C (24.1 %), and 10-methyl C (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family , a novel genus and species, gen. nov., sp. nov. are proposed. The type strain of is MUSC 201 ( = DSM 27763 = MCCC 1A00646 = NBRC 109973).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058701-0
2014-05-01
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1461.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058701-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. . ( 1993; ). Handbook of Microbiological Media. Edited by Parks L. C. . . Boca Raton:: CRC Press;.
    [Google Scholar]
  2. Carrillo P. G. , Mardaraz C. , Pitta-Alvarez S. I. , Giulietti A. M. . ( 1996; ). Isolation and selection of biosurfactant-producing bacteria. . World J Microbiol Biotechnol 12:, 82–84. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cerny G. . ( 1978; ). Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5:, 113–122. [CrossRef]
    [Google Scholar]
  5. Cho C. H. , Lee J.-S. , An D.-S. , Whon T. W. , Kim S.-G. . ( 2010; ). Nocardioides panacisoli sp. nov., isolated from the soil of a ginseng field. . Int J Syst Evol Microbiol 60:, 387–392. [CrossRef] [PubMed]
    [Google Scholar]
  6. Collins M. D. , Dorsch M. , Stackebrandt E. . ( 1989; ). Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov.. Int J Syst Bacteriol 39:, 1–6. [CrossRef]
    [Google Scholar]
  7. Dastager S. G. , Lee J.-C. , Ju Y.-J. , Park D.-J. , Kim C.-J. . ( 2008; ). Marmoricola bigeumensis sp. nov., a member of the family Nocardioidaceae . . Int J Syst Evol Microbiol 58:, 1060–1063. [CrossRef] [PubMed]
    [Google Scholar]
  8. Dastager S. G. , Lee J.-C. , Ju Y.-J. , Park D.-J. , Kim C.-J. . ( 2009; ). Nocardioides sediminis sp. nov., isolated from a sediment sample. . Int J Syst Evol Microbiol 59:, 280–284. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  10. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–789. [CrossRef]
    [Google Scholar]
  11. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  12. Hong K. , Gao A. H. , Xie Q. Y. , Gao H. , Zhuang L. , Lin H. P. , Yu H. P. , Li J. , Yao X. S. . & other authors ( 2009; ). Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. . Mar Drugs 7:, 24–44. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kaewkla O. , Franco C. M. M. . ( 2011; ). Flindersiella endophytica gen. nov., sp. nov., an endophytic actinobacterium isolated from the root of Grey Box, an endemic eucalyptus tree. . Int J Syst Evol Microbiol 61:, 2135–2140. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kates M. . ( 1986; ). Techniques of Lipidology, , 2nd edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  15. Kelly K. L. . ( 1964; ). Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  16. Kim S. H. , Yang H. O. , Sohn Y. C. , Kwon H. C. . ( 2010; ). Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi . . Int J Syst Evol Microbiol 60:, 2793–2798. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  19. Kuster E. , Williams S. T. . ( 1964; ). Selection of media for isolation of streptomycetes. . Nature 202:, 928–929. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lee D. W. , Lee S. D. . ( 2008; ). Aeromicrobium ponti sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 58:, 987–991. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lee D. W. , Lee S. D. . ( 2010; ). Marmoricola scoriae sp. nov., isolated from volcanic ash. . Int J Syst Evol Microbiol 60:, 2135–2139. [CrossRef] [PubMed]
    [Google Scholar]
  22. Lee S. D. , Lee D. W. , Ko Y.-H. . ( 2011; ). Marmoricola korecus sp. nov.. Int J Syst Evol Microbiol 61:, 1628–1631. [CrossRef] [PubMed]
    [Google Scholar]
  23. MacFaddin, J. F. (2000). Biochemical Tests for Identification of Medical Bacteria, 3rd edn. Baltimore: Lippincott, Williams & Wilkins.
  24. Meena B. , Rajan L. A. , Vinithkumar N. V. , Kirubagaran R. . ( 2013; ). Novel marine actinobacteria from emerald Andaman & Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts. . BMC Microbiol 13:, 145. [CrossRef] [PubMed]
    [Google Scholar]
  25. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  26. Miller E. S. , Woese C. R. , Brenner S. . ( 1991; ). Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov.. Int J Syst Bacteriol 41:, 363–368. [CrossRef] [PubMed]
    [Google Scholar]
  27. Nesterenko O. A. , Kvasnikov E. I. , Nogina T. M. . ( 1985; ). Nocardioidaceae fam. nov., a new family of the order Actinomycetales Buchanan 1917. . Mikrobiol Zh 47:, 3–12.
    [Google Scholar]
  28. Nesterenko O. A. , Kvasnikov E. I. , Nogina T. M. . ( 1990; ). Nocardioidaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 34. . Int J Syst Bacteriol 40:, 320–321. [CrossRef]
    [Google Scholar]
  29. O’Donnell A. G. , Goodfellow M. , Minnikin D. E. . ( 1982; ). Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) lochhead in the genus Nocardioides (Prauser) emend. O’Donnell et al. as Nocardioides simplex comb. nov.. Arch Microbiol 133:, 323–329. [CrossRef] [PubMed]
    [Google Scholar]
  30. Park Y. H. , Yoon J. H. , Shin Y. K. , Suzuki K. , Kudo T. , Seino A. , Kim H. J. , Lee J. S. , Lee S. T. . ( 1999; ). Classification of ‘Nocardioides fulvus’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov.. Int J Syst Bacteriol 49:, 743–752. [CrossRef] [PubMed]
    [Google Scholar]
  31. Prauser H. . ( 1976; ). Nocardioides, a new genus of the order Actinomycetales . . Int J Syst Bacteriol 26:, 58–65. [CrossRef]
    [Google Scholar]
  32. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  33. Sasser M . . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  34. Schleifer K. H. . ( 1985; ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  35. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  36. Schumann P. . ( 2011; ). Peptidoglycan structure. . Methods Microbiol 38:, 101–129. [CrossRef]
    [Google Scholar]
  37. Shieh W. Y. , Chen Y.-W. , Chaw S.-M. , Chiu H.-H. . ( 2003; ). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. . Int J Syst Evol Microbiol 53:, 479–484. [CrossRef] [PubMed]
    [Google Scholar]
  38. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  39. Sohn K. , Hong S. G. , Bae K. S. , Chun J. . ( 2003; ). Transfer of Hongia koreensis Lee et al. 2000 to the genus Kribbella Park et al. 1999 as Kribbella koreensis comb. nov.. Int J Syst Evol Microbiol 53:, 1005–1007. [CrossRef] [PubMed]
    [Google Scholar]
  40. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  41. Tamura T. , Yokota A. . ( 1994; ). Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov.. Int J Syst Bacteriol 44:, 608–611. [CrossRef]
    [Google Scholar]
  42. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  43. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  44. Urzì C. , Salamone P. , Schumann P. , Stackebrandt E. . ( 2000; ). Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. . Int J Syst Evol Microbiol 50:, 529–536. [CrossRef] [PubMed]
    [Google Scholar]
  45. Wang Y. M. , Zhang Z. S. , Xu X. L. , Ruan J. S. , Wang Y. . ( 2001; ). Actinopolymorpha singaporensis gen. nov., sp. nov., a novel actinomycete from the tropical rainforest of Singapore. . Int J Syst Evol Microbiol 51:, 467–473.[PubMed] [CrossRef]
    [Google Scholar]
  46. Whiton R. S. , Lau P. , Morgan S. L. , Gilbart J. , Fox A. . ( 1985; ). Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. . J Chromatogr A 347:, 109–120. [CrossRef] [PubMed]
    [Google Scholar]
  47. Yabe S. , Aiba Y. , Sakai Y. , Hazaka M. , Yokota A. . ( 2011; ). Thermasporomyces composti gen. nov., sp. nov., a thermophilic actinomycete isolated from compost. . Int J Syst Evol Microbiol 61:, 86–90. [CrossRef] [PubMed]
    [Google Scholar]
  48. Zhi X. Y. , Li W. J. , Stackebrandt E. . ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59:, 589–608. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.058701-0
Loading
/content/journal/ijsem/10.1099/ijs.0.058701-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error