1887

Abstract

A novel actinobacterial strain, designated MUSC 201, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201 represented a novel lineage within the class . Strain MUSC 201 formed a distinct clade in the family and was most closely related to the members of the genera (16S rRNA gene sequence similarity, 91.9–95.1 %), (92.7–94.6 %), (92.5–93.1 %) and (91.5–92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained -diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3γ. The peptidoglycan cell wall contained -diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5 : 0.9 : 1.0 : 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were Cω9 (30.8 %), C (24.1 %), and 10-methyl C (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family , a novel genus and species, gen. nov., sp. nov. are proposed. The type strain of is MUSC 201 ( = DSM 27763 = MCCC 1A00646 = NBRC 109973).

Funding
This study was supported by the:
  • , University of Malaya , (Award H-50001-A000027)
  • , Biotek Abadi , (Award GBA-808138)
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2014-05-01
2020-10-31
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