@article{mbs:/content/journal/ijsem/10.1099/ijs.0.058701-0, author = "Lee, Learn-Han and Zainal, Nurullhudda and Azman, Adzzie-Shazleen and Mutalib, Nurul-Syakima Ab and Hong, Kui and Chan, Kok-Gan", title = "Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_5", pages = "1461-1467", doi = "https://doi.org/10.1099/ijs.0.058701-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.058701-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel actinobacterial strain, designated MUSC 201T, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201T represented a novel lineage within the class Actinobacteria . Strain MUSC 201T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9–95.1 %), Aeromicrobium (92.7–94.6 %), Marmoricola (92.5–93.1 %) and Kribbella (91.5–92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained ll-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3γ. The peptidoglycan cell wall contained ll-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5 : 0.9 : 1.0 : 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C18 : 1ω9c (30.8 %), C16 : 0 (24.1 %), and 10-methyl C18 : 0 (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae , a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201T ( = DSM 27763T = MCCC 1A00646T = NBRC 109973T).", }