1887

Abstract

A novel strictly anaerobic, halotolerant, organotrophic bacterium, strain P3M-3, was isolated from a microbial mat formed under the flow of hot water emerging from a 2775 m-deep well in Tomsk region (western Siberia, Russia). Cells of strain P3M-3 were straight and curved rods, 0.2–0.4 µm in width and 1.5–20 µm in length. Strain P3M-3 grew optimally at 37 °C, pH 7.0–7.5 and in a NaCl concentration of 15 g l. Under optimum growth conditions, the doubling time was 1 h. The isolate was able to ferment a variety of mono-, di- and polysaccharides, including microcrystalline cellulose. Acetate, ethanol, H and CO were the main products of glucose fermentation. The DNA G+C content was 33.4 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain P3M-3 was a member of family , whose representatives are also found in cluster XIVa. 16S rRNA gene sequence similarity with HY-35-12, the closest relative, was 93.9 %. A novel genus and species, gen. nov., sp. nov., are proposed based on phylogenetic analysis and physiological properties of the novel isolate. The type strain of the type species is P3M-3 ( = DSM 26468 = VKM B-2804).

Funding
This study was supported by the:
  • , Federal Ministry of Education and Science , (Award 14.B37.21.1489 and 14.512.11.0070)
  • , RFBR , (Award KO_a_13-04-92606 and A_13-04-02157)
  • , Russian Academy of Sciences
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2014-08-01
2021-01-25
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References

  1. Bonch-Osmolovskaya E. A., Sokolova T. G., Kostrikina N. A., Zavarzin G. A. ( 1990 ). Desulfurella acetivorans gen. nov. and sp. nov. – a new thermophilic sulfur-reducing eubacterium. . Arch Microbiol 153, 151155. [CrossRef]
    [Google Scholar]
  2. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. ( 1994 ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. . Int J Syst Bacteriol 44, 812826. [CrossRef] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  4. Hardman J. K., Stadtman T. C. ( 1960 ). Metabolism of ω-acids. II. Fermentation of Δ-aminovaleric acid by Clostridium aminovalericum n. sp.. J Bacteriol 79, 549552.[PubMed]
    [Google Scholar]
  5. Jeong H., Yi H., Sekiguchi Y., Muramatsu M., Kamagata Y., Chun J. ( 2004 ). Clostridium jejuense sp. nov., isolated from soil. . Int J Syst Evol Microbiol 54, 14651468. [CrossRef] [PubMed]
    [Google Scholar]
  6. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  7. Larsen H. ( 1986 ). Halophilic and halotolerant microorganisms-an overview and historical perspective. . FEMS Microbiol Rev 39, 37. [CrossRef]
    [Google Scholar]
  8. Lovley D. R., Phillips E. J. P. ( 1988 ). Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. . Appl Environ Microbiol 54, 14721480.[PubMed]
    [Google Scholar]
  9. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  10. Mechichi T., Labat M., Garcia J. L., Thomas P., Patel B. K. C. ( 1999 ). Characterization of a new xylanolytic bacterium, Clostridium xylanovorans sp. nov.. Syst Appl Microbiol 22, 366371. [CrossRef] [PubMed]
    [Google Scholar]
  11. Miroshnichenko M. L., Kublanov I. V., Kostrikina N. A., Tourova T. P., Kolganova T. V., Birkeland N. K., Bonch-Osmolovskaya E. A. ( 2008 ). Caldicellulosiruptor kronotskyensis sp. nov. and Caldicellulosiruptor hydrothermalis sp. nov., two extremely thermophilic, cellulolytic, anaerobic bacteria from Kamchatka thermal springs. . Int J Syst Evol Microbiol 58, 14921496. [CrossRef] [PubMed]
    [Google Scholar]
  12. Park D. ( 2007 ). Genomic DNA isolation from different biological materials. . In Methods in Molecular Biology, ver. 353: Protocols for Nucleic Acid Analysis by Nonradioactive Probes, , 2nd edn., pp. 313. Edited by Hilario E., Mackay J. . Totowa, NJ:: Humana Press Inc;. [CrossRef]
    [Google Scholar]
  13. Perevalova A. A., Kublanov I. V., Baslerov R. V., Zhang G., Bonch-Osmolovskaya E. A. ( 2013 ). Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring. . Int J Syst Evol Microbiol 63, 479483. [CrossRef] [PubMed]
    [Google Scholar]
  14. Podosokorskaya O. A., Merkel A. Yu., Kolganova T. V., Chernyh N. A., Miroshnichenko M. L., Bonch-Osmolovskaya E. A., Kublanov I. V. ( 2011 ). Fervidobacterium riparium sp. nov., a thermophilic anaerobic cellulolytic bacterium isolated from a hot spring. . Int J Syst Evol Microbiol 61, 26972701. [CrossRef] [PubMed]
    [Google Scholar]
  15. Podosokorskaya O. A., Bonch-Osmolovskaya E. A., Novikov A. A., Kolganova T. V., Kublanov I. V. ( 2013a ). Ornatilinea apprima gen. nov., sp. nov., a cellulolytic representative of the class Anaerolineae . . Int J Syst Evol Microbiol 63, 8692. [CrossRef] [PubMed]
    [Google Scholar]
  16. Podosokorskaya O. A., Kadnikov V. V., Gavrilov S. N., Mardanov A. V., Merkel A. Y., Karnachuk O. V., Ravin N. V., Bonch-Osmolovskaya E. A., Kublanov I. V. ( 2013b ). Characterization of Melioribacter roseus gen. nov. sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae . . Environ Microbiol 15, 17591771. [CrossRef] [PubMed]
    [Google Scholar]
  17. Rainey F. A. ( 2009a ). Order I. Clostridiales Prévot 1953. . In Bergey’s Manual of Systematic Bacteriology , , 2nd edn., vol. 3 (The Firmicutes), p. 736. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  18. Rainey F. A. ( 2009b ). Family V. Lachnospiraceae fam. nov. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3 (The Firmicutes), p. 921. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  19. Rainey F. A., Hollen B. J. , Small A. ( 2009 ). Genus I. Clostridium Prazmowski 1880, 23AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3 (The Firmicutes), p. 738828. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  21. Slobodkina G. B., Panteleeva A. N., Kostrikina N. A., Kopitsyn D. S., Bonch-Osmolovskaya E. A., Slobodkin A. I. ( 2013 ). Tepidibacillus fermentans gen. nov., sp. nov.: a moderately thermophilic anaerobic and microaerophilic bacterium from an underground gas storage. . Extremophiles 17, 833839. [CrossRef] [PubMed]
    [Google Scholar]
  22. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607651. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Tamura K., Nei M. ( 1993 ). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. . Mol Biol Evol 10, 512526.[PubMed]
    [Google Scholar]
  24. Tamura K., Nei M., Kumar S. ( 2004 ). Prospects for inferring very large phylogenies by using the neighbor-joining method. . Proc Natl Acad Sci U S A 101, 1103011035. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  26. Trueper H. G., Schlegel H. G. ( 1964 ). Sulfur metabolism in Thiorhodaceae I. Quantitative measurements on growing cells of Chromatium okenii . . Antonie van Leeuwenhoek 30, 225238. [CrossRef] [PubMed]
    [Google Scholar]
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