1887

Abstract

A novel strictly anaerobic, halotolerant, organotrophic bacterium, strain P3M-3, was isolated from a microbial mat formed under the flow of hot water emerging from a 2775 m-deep well in Tomsk region (western Siberia, Russia). Cells of strain P3M-3 were straight and curved rods, 0.2–0.4 µm in width and 1.5–20 µm in length. Strain P3M-3 grew optimally at 37 °C, pH 7.0–7.5 and in a NaCl concentration of 15 g l. Under optimum growth conditions, the doubling time was 1 h. The isolate was able to ferment a variety of mono-, di- and polysaccharides, including microcrystalline cellulose. Acetate, ethanol, H and CO were the main products of glucose fermentation. The DNA G+C content was 33.4 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain P3M-3 was a member of family , whose representatives are also found in cluster XIVa. 16S rRNA gene sequence similarity with HY-35-12, the closest relative, was 93.9 %. A novel genus and species, gen. nov., sp. nov., are proposed based on phylogenetic analysis and physiological properties of the novel isolate. The type strain of the type species is P3M-3 ( = DSM 26468 = VKM B-2804).

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2014-08-01
2020-01-29
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