1887

Abstract

The taxonomic position of a novel actinomycete, strain RC 1831, isolated from the sediment of a fish dumping yard at Barkul village near Chilika Lake, Odisha, India, was determined by a polyphasic approach. Based on morphological and chemotaxonomic characteristics the isolate was determined to belong to the genus . The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1428 nt) with representative strains showed that the strain consistently falls into a distinct phyletic line together with DSM 41689 (98.22 % similarity) and a subclade consisting of DSM 42065 (98.40 %), R97 DSM 41901 (98.27 %), GIMN4.003 (98.33 %), DSM 41926 (98.13 %), NBRC 14749 (97.37 %) and NBRC 14748 (98.22 %). However, the levels of DNA–DNA relatedness between strain RC 1831 and phylogenetically related strains DSM 42065 (28.75±3.25 %) and DSM 41689 (15±2.40 %) were significantly lower than the 70 % threshold value for delineation of genomic species. Furthermore, the isolate could be distinguished phenotypically on the basis of physiological, morphological and biochemical differences from its closest phylogenetic neighbours and other related reference strains. Strain RC 1831 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RC 1831 ( = JCM 18754 = DSM 42082).

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2014-04-01
2019-10-17
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