RT Journal Article SR Electronic(1) A1 Ray, Lopamudra A1 Mishra, Samir Ranjan A1 Panda, Ananta Narayan A1 Rastogi, Gurdeep A1 Pattanaik, Ajit Kumar A1 Adhya, Tapan Kumar A1 Suar, Mrutyunjay A1 Raina, VishakhaYR 2014 T1 Streptomyces barkulensis sp. nov., isolated from an estuarine lake JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_4 SP 1365 OP 1372 DO https://doi.org/10.1099/ijs.0.056614-0 PB Microbiology Society, SN 1466-5034, AB The taxonomic position of a novel actinomycete, strain RC 1831T, isolated from the sediment of a fish dumping yard at Barkul village near Chilika Lake, Odisha, India, was determined by a polyphasic approach. Based on morphological and chemotaxonomic characteristics the isolate was determined to belong to the genus Streptomyces . The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1428 nt) with representative strains showed that the strain consistently falls into a distinct phyletic line together with Streptomyces glaucosporus DSM 41689T (98.22 % similarity) and a subclade consisting of Streptomyces atacamensis DSM 42065T (98.40 %), Streptomyces radiopugnans R97 DSM 41901T (98.27 %), Streptomyces fenghuangensis GIMN4.003T (98.33 %), Streptomyces nanhaiensis DSM 41926T (98.13 %), Streptomyces megasporus NBRC 14749T (97.37 %) and Streptomyces macrosporus NBRC 14748T (98.22 %). However, the levels of DNA–DNA relatedness between strain RC 1831T and phylogenetically related strains Streptomyces atacamensis DSM 42065T (28.75±3.25 %) and Streptomyces glaucosporus DSM 41689T (15±2.40 %) were significantly lower than the 70 % threshold value for delineation of genomic species. Furthermore, the isolate could be distinguished phenotypically on the basis of physiological, morphological and biochemical differences from its closest phylogenetic neighbours and other related reference strains. Strain RC 1831T is therefore considered to represent a novel species of the genus Streptomyces , for which the name Streptomyces barkulensis sp. nov. is proposed. The type strain is RC 1831T ( = JCM 18754T = DSM 42082T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.056614-0