1887

Abstract

Daqu is a fermentation starter used in the production of Chinese liquors. A thermophilic bacterium, designated strain H-18, was isolated from a high-temperature Daqu sample collected from the manufacturing process of a sesame-flavoured liquor in Shandong province, China. It was investigated in a taxonomic study using a polyphasic approach. Strain H-18 formed white aerial mycelium and greyish-yellow substrate mycelium, bearing single endospores on aerial and substrate hyphae or on unbranched short sporophores. The cell-wall peptidoglycan contained -diaminopimelic acid. The major fatty acids were iso-C and iso-C. The predominant menaquinone was MK-7. These chemotaxonomic properties are similar to those of members of the genus . The G+C content of the genomic DNA was 49.1 mol%. 16S rRNA gene sequence comparisons indicated that strain H-18 was most closely related to KCTC 9076 (96.42 % similarity), KCTC 9646 (96.06 %), KCTC 3666 (96.32 %) and KCTC 9790 (95.55 %). Strain H-18 showed low DNA–DNA relatedness (40.8, 33.4, 20.0 and 14.4 %) with the above strains. Based on morphological and chemotaxonomic characteristics, DNA–DNA hybridization data and physiological properties, strain H-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H-18 ( = DSM 45914 = CICC 10681).

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2014-01-01
2019-10-23
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