%0 Journal Article %A Yao, Su %A Liu, Yang %A Zhang, Mingjuan %A Zhang, Xin %A Li, Hong %A Zhao, Ting %A Xin, Chunhui %A Xu, Ling %A Zhang, Bolin %A Cheng, Chi %T Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature Daqu %D 2014 %J International Journal of Systematic and Evolutionary Microbiology, %V 64 %N Pt_1 %P 206-210 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.055509-0 %I Microbiology Society, %X Daqu is a fermentation starter used in the production of Chinese liquors. A thermophilic bacterium, designated strain H-18T, was isolated from a high-temperature Daqu sample collected from the manufacturing process of a sesame-flavoured liquor in Shandong province, China. It was investigated in a taxonomic study using a polyphasic approach. Strain H-18T formed white aerial mycelium and greyish-yellow substrate mycelium, bearing single endospores on aerial and substrate hyphae or on unbranched short sporophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major fatty acids were iso-C15 : 0 and iso-C17 : 0. The predominant menaquinone was MK-7. These chemotaxonomic properties are similar to those of members of the genus Thermoactinomyces . The G+C content of the genomic DNA was 49.1 mol%. 16S rRNA gene sequence comparisons indicated that strain H-18T was most closely related to Thermoactinomyces vulgaris KCTC 9076T (96.42 % similarity), Thermoactinomyces intermedius KCTC 9646T (96.06 %), Laceyella putida KCTC 3666T (96.32 %) and Laceyella sacchari KCTC 9790T (95.55 %). Strain H-18T showed low DNA–DNA relatedness (40.8, 33.4, 20.0 and 14.4 %) with the above strains. Based on morphological and chemotaxonomic characteristics, DNA–DNA hybridization data and physiological properties, strain H-18T represents a novel species of the genus Thermoactinomyces , for which the name Thermoactinomyces daqus sp. nov. is proposed. The type strain is H-18T ( = DSM 45914T = CICC 10681T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.055509-0