1887

Abstract

A newly isolated facultatively methylotrophic bacterium (strain 3t) was investigated. Cells of the isolate were Gram-stain-negative, asporogenous, non-motile rods that multiplied by binary fission. The strain utilized methanol, methylamine and a variety of multicarbon compounds as carbon and energy sources. Growth occurred at pH 6.5–8.5 (optimally at 7.0–7.5) and at 10–45 °C (optimally at 30–37 °C). The major fatty acids of methanol-grown cells were Cω7 and C. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. The major ubiquinone was Q-8. Strain 3t possessed pyrroloquinoline quinone (PQQ)-linked methanol dehydrogenase and assimilated C units at the level of formaldehyde and CO via the serine cycle. The DNA G+C content of the strain was 63.6 mol% ( ). On the basis of 16S rRNA gene sequence similarity (98.1 %) and rather low DNA–DNA relatedness (30 %) with the type strain of the type species of the genus ( FAM5), and physiological and biochemical characteristics, the isolate was classified as a representative of a new species of the genus and named 3t ( = VKM B-2692 = CCUG 61694 = DSM 25156).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055046-0
2014-01-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/1/158.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055046-0&mimeType=html&fmt=ahah

References

  1. Anthony C., Williams P.. ( 2003;). The structure and mechanism of methanol dehydrogenase. . Biochim Biophys Acta 1647:, 18–23. [CrossRef][PubMed]
    [Google Scholar]
  2. Baytshtok V., Lu H., Park H., Kim S., Yu R., Chandran K.. ( 2009;). Impact of varying electron donors on the molecular microbial ecology and biokinetics of methylotrophic denitrifying bacteria. . Biotechnol Bioeng 102:, 1527–1536. [CrossRef][PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  4. Doronina N. V., Braus-Stromeyer S. A., Leisinger T., Trotsenko Y. A.. ( 1995;). Isolation and characterization of a new facultatively methylotrophic bacterium: description of Methylorhabdus multivorans gen. nov., sp. nov.. Syst Appl Microbiol 18:, 92–98. [CrossRef]
    [Google Scholar]
  5. Doronina N. V., Trotsenko Y. A., Krausova V. I., Boulygina E. S., Tourova T. P.. ( 1998;). Methylopila capsulata gen. nov., sp. nov., a novel non-pigmented aerobic facultatively methylotrophic bacterium. . Int J Syst Bacteriol 48:, 1313–1321. [CrossRef][PubMed]
    [Google Scholar]
  6. Fedorov D. N., Ivanova E. G., Doronina N. V., Trotsenko IuA.. ( 2008;). [A new system of degenerate-oligonucleotide primers for detection and amplification of nifHD genes]. . Mikrobiologiia 77:, 286–288 (in Russian).[PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1989;). phylip – phylogeny inference package (version 3.2). . Cladistics 5:, 164–166.
    [Google Scholar]
  8. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  9. Kalyuzhnaya M. G., De Marco P., Bowerman S., Pacheco C. C., Lara J. C., Lidstrom M. E., Chistoserdova L.. ( 2006;). Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates. . Int J Syst Evol Microbiol 56:, 2517–2522. [CrossRef][PubMed]
    [Google Scholar]
  10. Kates M.. ( 1972;). Techniques of Lipidology. New York:: Elsevier;.
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Kittichotirat W., Good N. M., Hall R., Bringel F., Lajus A., Médigue C., Smalley N. E., Beck D., Bumgarner R.. & other authors ( 2011;). Genome sequence of Methyloversatilis universalis FAM5T, a methylotrophic representative of the order Rhodocyclales. . J Bacteriol 193:, 4541–4542. [CrossRef][PubMed]
    [Google Scholar]
  13. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J.. ( 1951;). Protein measurement with the Folin phenol reagent. . J Biol Chem 193:, 265–275.[PubMed]
    [Google Scholar]
  15. Lu H., Kalyuzhnaya M. G., Chandran K.. ( 2012;). Comparative proteomic analysis reveals insights into anoxic growth of Methyloversatilis universalis FAM5 on methanol and ethanol. . Environ Microbiol 14:, 2935–2945. [CrossRef][PubMed]
    [Google Scholar]
  16. Marmur J. A.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–214. [CrossRef]
    [Google Scholar]
  17. McDonald I. R., Murrell J. C.. ( 1997;). The methanol dehydrogenase structural gene mxaF and its use as a functional gene probe for methanotrophs and methylotrophs. . Appl Environ Microbiol 63:, 3218–3224.[PubMed]
    [Google Scholar]
  18. Owen R. J., Lapage S. P.. ( 1976;). The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. . J Appl Bacteriol 41:, 335–340. [CrossRef][PubMed]
    [Google Scholar]
  19. Sambrook J., Russell D. W.. ( 2001;). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  20. Sasser M.., ( 1990;). Identification of bacteria by gas chromatography of fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  22. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  23. Trotsenko Y. A., Doronina N. V., Govorukhina N. I.. ( 1986;). Metabolism of non-motile obligately methylotrophic bacteria. . FEMS Microbiol Lett 33:, 293–297. [CrossRef]
    [Google Scholar]
  24. Van de Peer Y., De Wachter R.. ( 1994;). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. . Comput Appl Biosci 10:, 569–570.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.055046-0
Loading
/content/journal/ijsem/10.1099/ijs.0.055046-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error