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Abstract

A newly isolated facultatively methylotrophic bacterium (strain 3t) was investigated. Cells of the isolate were Gram-stain-negative, asporogenous, non-motile rods that multiplied by binary fission. The strain utilized methanol, methylamine and a variety of multicarbon compounds as carbon and energy sources. Growth occurred at pH 6.5–8.5 (optimally at 7.0–7.5) and at 10–45 °C (optimally at 30–37 °C). The major fatty acids of methanol-grown cells were Cω7 and C. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. The major ubiquinone was Q-8. Strain 3t possessed pyrroloquinoline quinone (PQQ)-linked methanol dehydrogenase and assimilated C units at the level of formaldehyde and CO via the serine cycle. The DNA G+C content of the strain was 63.6 mol% ( ). On the basis of 16S rRNA gene sequence similarity (98.1 %) and rather low DNA–DNA relatedness (30 %) with the type strain of the type species of the genus ( FAM5), and physiological and biochemical characteristics, the isolate was classified as a representative of a new species of the genus and named 3t ( = VKM B-2692 = CCUG 61694 = DSM 25156).

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2014-01-01
2021-01-15
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