@article{mbs:/content/journal/ijsem/10.1099/ijs.0.055046-0, author = "Doronina, Nina V. and Kaparullina, Elena N. and Trotsenko, Yuri A.", title = "Methyloversatilis thermotolerans sp. nov., a novel thermotolerant facultative methylotroph isolated from a hot spring", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_1", pages = "158-164", doi = "https://doi.org/10.1099/ijs.0.055046-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.055046-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A newly isolated facultatively methylotrophic bacterium (strain 3tT) was investigated. Cells of the isolate were Gram-stain-negative, asporogenous, non-motile rods that multiplied by binary fission. The strain utilized methanol, methylamine and a variety of multicarbon compounds as carbon and energy sources. Growth occurred at pH 6.5–8.5 (optimally at 7.0–7.5) and at 10–45 °C (optimally at 30–37 °C). The major fatty acids of methanol-grown cells were C16 : 1ω7c and C16 : 0. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. The major ubiquinone was Q-8. Strain 3tT possessed pyrroloquinoline quinone (PQQ)-linked methanol dehydrogenase and assimilated C1 units at the level of formaldehyde and CO2 via the serine cycle. The DNA G+C content of the strain was 63.6 mol% (T m). On the basis of 16S rRNA gene sequence similarity (98.1 %) and rather low DNA–DNA relatedness (30 %) with the type strain of the type species of the genus Methyloversatilis ( Methyloversatilis universalis FAM5T), and physiological and biochemical characteristics, the isolate was classified as a representative of a new species of the genus and named Methyloversatilis thermotolerans 3tT ( = VKM B-2692T = CCUG 61694T = DSM 25156T).", }