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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_11

sp. nov., an actinobacterium isolated from (Lour.) Benth Free

Abstract

A novel endophytic actinobacterium, designated strain YIM 67072, was isolated from healthy roots of (Lour.) Benth. Cells of this aerobic, cream–yellow-coloured strain occurred singly, in pairs or in tetrads, were Gram-stain-positive and ovoid- to spherical-shaped. Strain YIM 67072 grew at 4–45 °C, pH 5.0–10.0 and in the presence of 0–7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 67072 belonged to the genus . The isolate contained MK-7 as the major component of the quinone system. The peptidoglycan type was A3α. The polar lipid profile consisted predominantly of diphosphatidylglycerol and glycolipids. The major fatty acids were anteiso-C, iso-C, anteiso-C and iso-C. The DNA G+C content was 53.2 mol%. However, strain YIM 67072 differed from its closest relatives CCUG 35957 (98.5 % 16S rRNA gene sequence similarity), JCM 11375 (97.6 %) and L-143 (97.3 %) in many phenotypic characteristics. Moreover, the levels of DNA–DNA relatedness between the novel isolate and the three above-mentioned type strains were 28.7±1.3 %, 36.5±1.2 %, 46.8±1.5 %, respectively. Based on comparative analysis of physiological and chemotaxonomic data, strain YIM 67072 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 67072 ( = DSM 26247 = JCM 18541).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award U0932601)
  • ‘Hundred Talents Program’ of the Chinese Academy of Sciences
© 2013 IUMS
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2013-11-01
2023-12-07
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Rothiaendophytica sp. nov., an actinobacterium isolated from Dysophylla stellata (Lour.) Benth
Int J Syst Evol Microbiol 63, 3964 (2013); https://doi.org/10.1099/ijs.0.052522-0
/content/journal/ijsem/10.1099/ijs.0.052522-0
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