%0 Journal Article %A Xiong, Zi-Jun %A Zhang, Jin-Li %A Zhang, Dao-Feng %A Zhou, Zhi-Li %A Liu, Min-Jiao %A Zhu, Wen-Yong %A Zhao, Li-Xing %A Xu, Li-Hua %A Li, Wen-Jun %T Rothiaendophytica sp. nov., an actinobacterium isolated from Dysophylla stellata (Lour.) Benth %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_11 %P 3964-3969 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.052522-0 %I Microbiology Society, %X A novel endophytic actinobacterium, designated strain YIM 67072T, was isolated from healthy roots of Dysophylla stellata (Lour.) Benth. Cells of this aerobic, cream–yellow-coloured strain occurred singly, in pairs or in tetrads, were Gram-stain-positive and ovoid- to spherical-shaped. Strain YIM 67072T grew at 4–45 °C, pH 5.0–10.0 and in the presence of 0–7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 67072T belonged to the genus Rothia . The isolate contained MK-7 as the major component of the quinone system. The peptidoglycan type was A3α. The polar lipid profile consisted predominantly of diphosphatidylglycerol and glycolipids. The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content was 53.2 mol%. However, strain YIM 67072T differed from its closest relatives Rothia nasimurium CCUG 35957T (98.5 % 16S rRNA gene sequence similarity), Rothia amarae JCM 11375T (97.6 %) and Rothia terrae L-143T (97.3 %) in many phenotypic characteristics. Moreover, the levels of DNA–DNA relatedness between the novel isolate and the three above-mentioned type strains were 28.7±1.3 %, 36.5±1.2 %, 46.8±1.5 %, respectively. Based on comparative analysis of physiological and chemotaxonomic data, strain YIM 67072T represents a novel species of the genus Rothia , for which the name Rothia endophytica sp. nov. is proposed. The type strain is YIM 67072T ( = DSM 26247T = JCM 18541T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.052522-0