1887

Abstract

Three pink-pigmented, aerobic, Gram-stain-negative, rod-shaped and facultatively methylotrophic strains were isolated from the phyllosphere of and . 16S rRNA gene sequence analysis support the affiliation of all strains to the genus . The closest relatives of strains C34 and T5 were 23e (98.0 and 98.5 % sequence similarity, respectively) and JCM 2833 (97.0 and 97.2 %, respectively). Strain TA73 showed the highest sequence similarities to JT1 and F3.2 (both 97.9 %), followed by CBMB27 and DSM 19569 (both 97.8 %), C15 and JCM 2831 (both 97.7 %). The major components in the fatty acid profiles were Cω7, C and one unknown fatty acid for strain TA73 and Cω7, Cω7/iso-C 2-OH, C and C for strains C34 and T5. Physiological and biochemical analysis, including DNA–DNA hybridization, revealed clear differences between the investigated strains and their closest phylogenetic neighbours. DNA–DNA hybridization studies also showed high similarities between strains C34 and T5 (59.6–100 %). Therefore, the isolates represent two novel species within the genus , for which the names sp. nov. (type strain TA73 = LMG 25778 = CCM 7786) and sp. nov. (type strain C34 = LMG 25777 = CCM 7787) are proposed.

Funding
This study was supported by the:
  • DFG Priority Program 1374 ‘Infrastructure-Biodiversity-Exploratories’ (Award Ka 875/6-1 to P. K.)
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2013-07-01
2021-08-01
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