@article{mbs:/content/journal/ijsem/10.1099/ijs.0.047787-0, author = "Wellner, S. and Lodders, N. and Glaeser, S. P. and Kämpfer, P.", title = "Methylobacteriumtrifolii sp. nov. and Methylobacteriumthuringiense sp. nov., methanol-utilizing, pink-pigmented bacteria isolated from leaf surfaces", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_7", pages = "2690-2699", doi = "https://doi.org/10.1099/ijs.0.047787-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.047787-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Three pink-pigmented, aerobic, Gram-stain-negative, rod-shaped and facultatively methylotrophic strains were isolated from the phyllosphere of Trifolium repens and Cerastium holosteoides. 16S rRNA gene sequence analysis support the affiliation of all strains to the genus Methylobacterium . The closest relatives of strains C34T and T5 were Methylobacterium gnaphalii 23eT (98.0 and 98.5 % sequence similarity, respectively) and Methylobacterium organophilum JCM 2833T (97.0 and 97.2 %, respectively). Strain TA73T showed the highest sequence similarities to Methylobacterium marchantiae JT1T and Methylobacterium bullatum F3.2T (both 97.9 %), followed by Methylobacterium phyllosphaerae CBMB27T and Methylobacterium brachiatum DSM 19569T (both 97.8 %), Methylobacterium cerastii C15T and Methylobacterium radiotolerans JCM 2831T (both 97.7 %). The major components in the fatty acid profiles were C18 : 1ω7c, C16 : 0 and one unknown fatty acid for strain TA73T and C18 : 1ω7c, C16 : 1ω7c/iso-C15 : 0 2-OH, C18 : 0 and C16 : 0 for strains C34T and T5. Physiological and biochemical analysis, including DNA–DNA hybridization, revealed clear differences between the investigated strains and their closest phylogenetic neighbours. DNA–DNA hybridization studies also showed high similarities between strains C34T and T5 (59.6–100 %). Therefore, the isolates represent two novel species within the genus Methylobacterium , for which the names Methylobacterium trifolii sp. nov. (type strain TA73T = LMG 25778T = CCM 7786T) and Methylobacterium thuringiense sp. nov. (type strain C34T = LMG 25777T = CCM 7787T) are proposed.", }