1887

Abstract

A newly isolated, facultatively methylotrophic bacterium (strain MUSA) was investigated. The isolate was strictly aerobic, Gram-stain-negative, asporogenous, motile, rod-shaped and multiplied by binary fission. The strain utilized methanol, methylamine and an apparently narrow range of multi-carbon compounds, but not methane, dichloromethane or CO/H, as the carbon and energy sources. Growth occurred at pH 5.5–9.5 (optimum, pH 7.0) and 16–40 °C (optimum, 28–30 °C). The major fatty acids of methanol-grown cells were Cω7, C and 11-methyl-Cω7 . The predominant phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. The major ubiquinone was Q-10. The strain had methanol and methylamine dehydrogenases as well as the enzymes of the -methylglutamate pathway (lyases of γ-glutamylmethylamide and -methylglutamate). C assimilation occurs via the isocitrate lyase-negative serine pathway. Ammonium was assimilated by glutamate dehydrogenase and the glutamate cycle (glutamate synthase/glutamine synthetase). The DNA G+C content of the strain was 64.5 mol% (determined from the melting temperature). Based on 16S rRNA gene sequence similarity (97.0–98.9 %) and DNA–DNA relatedness (36–38 %) with representatives of the genus ( IM1 and JZL-4) the isolate was classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MUSA ( = VKM B-2646 = DSM 24986 = CCUG 61696).

Funding
This study was supported by the:
  • , Russian Foundation for Basic Research (RFBR) , (Award 10-04-00808)
  • , RSC , (Award 14. 740. 11. 0111)
  • , Russian Foundation for Basic Research (RFBR) , (Award 10-04-00808)
  • , RSC , (Award 14. 740. 11. 0111)
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2013-05-01
2020-08-12
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