%0 Journal Article %A Doronina, Nina V. %A Kaparullina, Elena N. %A Bykova, Tatjana V. %A Trotsenko, Yuri A. %T Methylopila musalis sp. nov., an aerobic, facultatively methylotrophic bacterium isolated from banana fruit %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_5 %P 1847-1852 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.042028-0 %I Microbiology Society, %X A newly isolated, facultatively methylotrophic bacterium (strain MUSAT) was investigated. The isolate was strictly aerobic, Gram-stain-negative, asporogenous, motile, rod-shaped and multiplied by binary fission. The strain utilized methanol, methylamine and an apparently narrow range of multi-carbon compounds, but not methane, dichloromethane or CO2/H2, as the carbon and energy sources. Growth occurred at pH 5.5–9.5 (optimum, pH 7.0) and 16–40 °C (optimum, 28–30 °C). The major fatty acids of methanol-grown cells were C18 : 1ω7c, C18 : 0 and 11-methyl-C18 : 1ω7c . The predominant phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. The major ubiquinone was Q-10. The strain had methanol and methylamine dehydrogenases as well as the enzymes of the N-methylglutamate pathway (lyases of γ-glutamylmethylamide and N-methylglutamate). C1 assimilation occurs via the isocitrate lyase-negative serine pathway. Ammonium was assimilated by glutamate dehydrogenase and the glutamate cycle (glutamate synthase/glutamine synthetase). The DNA G+C content of the strain was 64.5 mol% (determined from the melting temperature). Based on 16S rRNA gene sequence similarity (97.0–98.9 %) and DNA–DNA relatedness (36–38 %) with representatives of the genus Methylopila ( Methylopila capsulata IM1T and Methylopila jiangsuensis JZL-4T) the isolate was classified as a novel species of the genus Methylopila , for which the name Methylopila musalis sp. nov. is proposed. The type strain is MUSAT ( = VKM B-2646T = DSM 24986T = CCUG 61696T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.042028-0