1887

Abstract

A Gram-negative, motile, aerobic, oxidase- and catalase-positive rod, designated P10-2-4, was isolated from an intertidal sand sample collected from a coastal area of Qingdao (Yellow Sea), China. The isolate reduced nitrate to nitrite and grew at 4–33 °C and with 0.5–12 % (w/v) NaCl. The predominant cellular fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH), Cω7 and C. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major respiratory quinone was Q-8. The genomic DNA G+C content was 45.1 %. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain P10-2-4 belonged to the genus . The isolate shared the highest 16S rRNA gene sequence similarity (98.1 %) with JAMM 0745 and 96.9, 96.5 and 95.9 % sequence similarities with S3-22, LHW37 and NAG-2N-126, respectively, strains of the other three recognized species in the genus. DNA–DNA relatedness between strain P10-2-4 and JCM 14595 was 35.6 %. Furthermore, strain P10-2-4 could be distinguished from the representatives of the genus by a combination of phenotypic characteristics, such as temperature and NaCl concentration for growth, nitrate reduction, DNase activity and assimilation of substrates. The data from this study suggests that strain P10-2-4 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is P10-2-4 ( = CGMCC 1.10971  = KCTC 23686).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41176130, 41106161, 31170055, 31070061 and 31025001)
  • Hi-Tech Research and Development program of China (Award 2012AA092103 and 2011AA090703)
  • Special Fund of China for Marine-scientific Research in the Public Interest (Award 201005032-6)
  • Natural Science Foundation of Shandong Province, China (Award ZR2009DZ002 and JQ200910)
  • Foundation for Young Excellent Scientists in Shandong Province, China (Award BS2010SW015)
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-T-05)
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2013-05-01
2021-10-23
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