%0 Journal Article %A Liu, Ang %A Zhang, Xi-Ying %A Chen, Chun-Xiao %A Xie, Bin-Bin %A Qin, Qi-Long %A Liu, Chang %A Li, Guo-Wei %A Li, Hai %A Xu, Zhong %A Chen, Xiu-Lan %A Zhou, Bai-Cheng %A Zhang, Yu-Zhong %T Neptunomonas qingdaonensis sp. nov., isolated from intertidal sand %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_5 %P 1673-1677 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.041970-0 %I Microbiology Society, %X A Gram-negative, motile, aerobic, oxidase- and catalase-positive rod, designated P10-2-4T, was isolated from an intertidal sand sample collected from a coastal area of Qingdao (Yellow Sea), China. The isolate reduced nitrate to nitrite and grew at 4–33 °C and with 0.5–12 % (w/v) NaCl. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C18 : 1ω7c and C16 : 0. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major respiratory quinone was Q-8. The genomic DNA G+C content was 45.1 %. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain P10-2-4T belonged to the genus Neptunomonas . The isolate shared the highest 16S rRNA gene sequence similarity (98.1 %) with Neptunomonas japonica JAMM 0745T and 96.9, 96.5 and 95.9 % sequence similarities with N. antarctica S3-22T, N. concharum LHW37T and N. naphthovorans NAG-2N-126T, respectively, strains of the other three recognized species in the genus. DNA–DNA relatedness between strain P10-2-4T and N. japonica JCM 14595T was 35.6 %. Furthermore, strain P10-2-4T could be distinguished from the representatives of the genus Neptunomonas by a combination of phenotypic characteristics, such as temperature and NaCl concentration for growth, nitrate reduction, DNase activity and assimilation of substrates. The data from this study suggests that strain P10-2-4T represents a novel species in the genus Neptunomonas , for which the name Neptunomonas qingdaonensis sp. nov. is proposed. The type strain is P10-2-4T ( = CGMCC 1.10971T  = KCTC 23686T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.041970-0