1887

Abstract

Two strains, designated Sac-22 and Sac-41, were isolated from rhizosphere soil and rhizoplane of field-grown sugar cane clone Co86032. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class , their closest relatives being and with 16S rRNA gene sequence pairwise similarities of 96.4–97.2 % to the two novel strains. Strains Sac-22 and Sac-41 shared a 16S rRNA gene sequence similarity value of 97.6 %. Cells of the two strains were Gram-reaction-negative, aerobic, motile and rod-shaped. Ubiquinone (Q-8) was the respiratory quinone and the predominant polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The main cellular fatty acids were C, Cω7/iso-C 2-OH, C cyclo, C 3-OH and C. The DNA GC content of the genomic DNA was 56.4 mol% for strain Sac-22 and 54.9 mol% for strain Sac-41. Based on the results of 16S rRNA gene sequence analysis and physiological and biochemical characterization, that differentiated strains Sac-22 and Sac-41 from all recognized species of the genus , it was concluded that strains represent two novel species in the genus for which the names sp. nov. (type strain Sac-22 = KCTC 22381 = NCIMB 14475) and sp. nov. (type strain Sac-41 = KCTC 22382 = NCIMB 14476) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.040584-0
2013-03-01
2021-02-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/1126.html?itemId=/content/journal/ijsem/10.1099/ijs.0.040584-0&mimeType=html&fmt=ahah

References

  1. Bozzola J. J., Russell L. D. ( 1998 ). Electron Microscopy, , 2nd edn.. Sudbury, MS:: Jones & Bartlett;.
    [Google Scholar]
  2. Chanprame S., Todd J. J., Widholm J. M. ( 1996 ). Prevention of pink-pigmented methylotrophic bacteria (Methylobacterium mesophilicum) contamination of plant tissue cultures. . Plant Cell Rep 16, 222225. [CrossRef]
    [Google Scholar]
  3. DeLong E. F. ( 1992 ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89, 56855689. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  6. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  7. Friedman B. A., Dugan P. R., Pfister R. M., Remsen C. C. ( 1968 ). Fine structure and composition of the zoogloeal matrix surrounding Zoogloea ramigera . . J Bacteriol 96, 21442153.[PubMed]
    [Google Scholar]
  8. Garrity G. M., Bell J. A., Lilburn T. ( 2005 ). The Proteobacteria, Part C, alpha, beta, delta and epsilon Proteobacteria. . In Bergey’s Manual of Systematic Bacteriology, , 2nd Edn., Vol. 2, p. 628. Edited by Brenner D. J., Krieg N. R., Staley J. T, Garrity G. M. . New York:, Springer;.
    [Google Scholar]
  9. Green P. N., Bousfield I. J. ( 1982 ). A taxonomic study of some Gram-negative facultatively methylotrophic bacteria. . Microbiol 128, 623638. [CrossRef]
    [Google Scholar]
  10. Hiraishi A., Shin Y. K., Sugiyama J. ( 1997 ). Proposal to reclassify Zoogloea ramigera IAM 12670 (P. R. Dugan 115) as Duganella zoogloeoides gen. nov., sp. nov.. Int J Syst Bacteriol 47, 12491252. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kämpfer P., Wellner S., Lohse K., Martin K., Lodders N. ( 2012 ). Duganella phyllosphaerae sp. nov., isolated from the leaf surface of Trifolium repens and proposal to reclassify Duganella violaceinigra into a novel genus as Pseudoduganella violceinigra gen. nov., comb. nov.. Syst Appl Microbiol 35, 1923. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Laurent P., Buchon L., Guespin-Michel J. F., Orange N. ( 2000 ). Production of pectate lyases and cellulases by Chryseomonas luteola strain MFCL0 depends on the growth temperature and the nature of the culture medium: evidence for two critical temperatures. . Appl Environ Microbiol 66, 15381543. [CrossRef] [PubMed]
    [Google Scholar]
  14. Li W. J., Zhang Y. Q., Park D. J., Li C. T., Xu L. H., Kim C. J., Jiang C. L. ( 2004 ). Duganella violaceinigra sp. nov., a novel mesophilic bacterium isolated from forest soil. . Int J Syst Evol Microbiol 54, 18111814. [CrossRef] [PubMed]
    [Google Scholar]
  15. Madhaiyan M., Poonguzhali S., Sa T. M. ( 2007b ). Metal tolerating methylotrophic bacteria reduces nickel and cadmium toxicity and promotes plant growth of tomato (Lycopersicon esculentum L). . Chemosphere 69, 220228. [CrossRef] [PubMed]
    [Google Scholar]
  16. Madhaiyan M., Kim B.-Y., Poonguzhali S., Kwon S.-W., Song M.-H., Ryu J.-H., Go S.-J., Koo B.-S., Sa T.-M. ( 2007c ). Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice. . Int J Syst Evol Microbiol 57, 326331. [CrossRef] [PubMed]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  19. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees.. Mol Biol Evol 4, 406425.
    [Google Scholar]
  21. Schwyn B., Neilands J. B. ( 1987 ). Universal chemical assay for the detection and determination of siderophores. . Anal Biochem 160, 4756. [CrossRef] [PubMed]
    [Google Scholar]
  22. Seldin L., Dubnau D. ( 1985 ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35, 151154. [CrossRef]
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Evol Bacteriol 44, 846849. [CrossRef]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tan Z. Y., Wang E. T., Peng G. X., Zhu M. E., Martínez-Romero E., Chen W. X. ( 1999 ). Characterization of bacteria isolated from wild legumes in the north-western regions of China. . Int J Syst Bacteriol 49, 14571469. [CrossRef] [PubMed]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [CrossRef] [PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the Ad Hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  28. Whittenbury R., Phillips K. C., Wilkinson J. F. ( 1970 ). Enrichment, isolation and some properties of methane-utilizing bacteria. . J Gen Microbiol 61, 205218. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.040584-0
Loading
/content/journal/ijsem/10.1099/ijs.0.040584-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error