@article{mbs:/content/journal/ijsem/10.1099/ijs.0.040584-0, author = "Madhaiyan, M. and Poonguzhali, S. and Saravanan, V. S. and Hari, K. and Lee, K.-C. and Lee, J.-S.", title = "Duganellasacchari sp. nov. and Duganellaradicis sp. nov., two novel species isolated from rhizosphere of field-grown sugar cane", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_3", pages = "1126-1131", doi = "https://doi.org/10.1099/ijs.0.040584-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.040584-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two strains, designated Sac-22T and Sac-41T, were isolated from rhizosphere soil and rhizoplane of field-grown sugar cane clone Co86032. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class Betaproteobacteria , their closest relatives being Pseudoduganella violaceinigra and Duganella zoogloeoides with 16S rRNA gene sequence pairwise similarities of 96.4–97.2 % to the two novel strains. Strains Sac-22T and Sac-41T shared a 16S rRNA gene sequence similarity value of 97.6 %. Cells of the two strains were Gram-reaction-negative, aerobic, motile and rod-shaped. Ubiquinone (Q-8) was the respiratory quinone and the predominant polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The main cellular fatty acids were C16 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH, C17 : 0 cyclo, C10 : 0 3-OH and C12 : 0. The DNA G+C content of the genomic DNA was 56.4 mol% for strain Sac-22T and 54.9 mol% for strain Sac-41T. Based on the results of 16S rRNA gene sequence analysis and physiological and biochemical characterization, that differentiated strains Sac-22T and Sac-41T from all recognized species of the genus Duganella , it was concluded that strains represent two novel species in the genus Duganella for which the names Duganella sacchari sp. nov. (type strain Sac-22T = KCTC 22381T = NCIMB 14475T) and Duganella radicis sp. nov. (type strain Sac-41T = KCTC 22382T = NCIMB 14476T) are proposed.", }