1887

Abstract

A group of nine Gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms of acute oak decline (AOD) in the UK were investigated using a polyphasic approach. 16S rRNA gene sequencing and phylogenetic analysis revealed that these isolates form a distinct lineage within the genus , family , and are most closely related to (97.6 % sequence similarity to the type strain). Multilocus sequence analysis based on four housekeeping genes (, , and ) confirmed their position within the genus , while DNA–DNA hybridization indicated that the isolates belong to a single taxon. The isolates can be differentiated phenotypically from their closest phylogenetic neighbours. The phylogenetic and phenotypic data demonstrate that these isolates from oak with symptoms of AOD represent a novel species in the genus , for which the name sp. nov. (type strain FRB 141  = R-43656  = BCC 845  = LMG 26270  = NCPPB 4484) is proposed.

Funding
This study was supported by the:
  • Forestry Commission, UK
  • Department of Science and Technology Centre of Excellence in Tree Health Biotechnology (DST CTHB), University of Pretoria, South Africa
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2012-10-01
2021-10-22
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