1887

Abstract

A novel endophytic actinomycete, designated strain YIM 68255, was isolated from healthy leaves of Decne. collected in Yunnan province, south-west China and characterized by using a polyphasic approach. The strain formed well-developed substrate mycelium, but no aerial mycelium. It grew at 10–45 °C, at pH 5–10 (optimum pH 7) and in the presence of 0–3 % (w/v) NaCl. The DNA G+C content was 73.0 mol%. Phylogenetic analyses showed that strain YIM 68255 belonged to the genus . However, it exhibited some differences from YIM 61359 and the level of DNA–DNA relatedness was 42.7±1.3 %. Based on comparative analysis of physiological and chemotaxonomic data, it is proposed that strain YIM 68255 represents a novel species of the genus , sp. nov., with strain YIM 68255 ( = DSM 45387 = CCTCC AA 209047) as the type strain. In addition, it is also proposed that Thawai 2010 be transferred to the genus as comb. nov. [type strain CM2-8 ( = JCM 15677 = BCC 34762)] based on chemotaxonomic characteristics and phylogenetic analysis. Emended descriptions of the genus and are also provided.

Funding
This study was supported by the:
  • , National Basic Research Program of China , (Award 2010CB833801)
  • , National Natural Science Foundation of China , (Award U0932601)
  • , Basic Research Foundation of Yunnan University , (Award 2010ZD001)
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2012-10-01
2021-03-09
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