1887

Abstract

An oval to rod-shaped, Gram-stain-negative, phototrophic bacterium, strain JA738, was isolated from a sediment sample collected from a pink pond. Strain JA738 was non-motile and had vesicular-type intracellular photosynthetic membranes. Bacteriochlorophyll and carotenoids of the spheroidene series were present as the major photosynthetic pigments. Strain JA738 required thiamine and pantothenate for growth. The major cellular fatty acids were Cω7, Cω5, C and Cω711-methyl; minor amounts of C 3-OH and C were also present. The major quinone was Q-10 and major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and two unidentified sulfolipids (SL1–2). Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA738 clustered with species of the genus in the class . Strain JA738 was most closely related to DSM 2781 (96.4 % 16S rRNA gene sequence similarity) and other members of the genus (<96.1 %). On the basis of phenotypic and molecular genetic evidence, it is proposed that strain JA738 should be classified as a novel species of the genus for which the name sp. nov. is proposed. The type strain is JA738 ( = DSM 24766 = KCTC 15110).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.036152-0
2012-10-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/10/2528.html?itemId=/content/journal/ijsem/10.1099/ijs.0.036152-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Britton G. , Liaaen-Jensen S. , Pfander H. . ( 2004; ). Carotenoids with a C40 skeleton. (a) hydrocarbons. . In Carotenoids Handbook, Main List 31, pp. 1–50. Edited by Britton G. , Liaaen-Jensen S. , Pfander H. . . Boston:: Birkhauser;.
    [Google Scholar]
  3. Brosius J. , Palmer M. L. , Kennedy P. J. , Noller H. F. . ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. Hanada S. , Takaichi S. , Matsuura K. , Nakamura K. . ( 2002; ). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. . Int J Syst Evol Microbiol 52:, 187–193.[PubMed] [CrossRef]
    [Google Scholar]
  6. Hiraishi A. , Hoshino Y. . ( 1984; ). Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. . J Gen Appl Microbiol 30:, 435–448. [CrossRef]
    [Google Scholar]
  7. Hiraishi A. , Ueda Y. . ( 1994; ). Intragenic structure of the gunus Rhodobacter: Transfer of Rhodobacter sulfidophilus and related marine species to the genus Rhodovulum gen. nov.. Int J Syst Bact 44:, 15–23. [CrossRef]
    [Google Scholar]
  8. Hiraishi A. , Hoshino Y. , Kitamura H. . ( 1984; ). Isoprenoid quinone composition in the classification of Rhodospirillaceae . . J Gen Appl Microbiol 30:, 197–210. [CrossRef]
    [Google Scholar]
  9. Imhoff J. F. , Pfennig N. . ( 2001; ). Thioflavicoccus mobilis gen. nov., sp. nov., a novel purple sulfur bacterium with bacteriochlorophyll b . . Int J Syst Evol Microbiol 51:, 105–110.[PubMed]
    [Google Scholar]
  10. Imhoff J. F. , Süling J. , Petri R. . ( 1998; ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . . Int J Syst Bacteriol 48:, 1129–1143. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kalyan Chakravarthy S. , Sucharitha K. , Sasikala Ch. , Ramana Ch. V. . ( 2009; ). Rhodovulum lacipunicei sp. nov., an obligate sulfide-demanding phototrophic alphaproteobacterium isolated from a purple pond in India. . Int J Syst Evol Microbiol 59:, 1615–1619. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kates M. . ( 1972; ). Techniques of Lipidology. New York:: Elsevier;.
    [Google Scholar]
  13. Kates M. . ( 1986; ). Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids. Amsterdam:: Elsevier;.
    [Google Scholar]
  14. Lakshmi K. V. N. S. , Sasikala Ch. , Ramana Ch. V. . ( 2009; ). Rhodoplanes pokkaliisoli sp. nov., a phototrophic alphaproteobacterium isolated from a waterlogged brackish paddy soil. . Int J Syst Evol Microbiol 59:, 2153–2157. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lakshmi K. V. N. S. , Sasikala Ch. , Ramana V. V. , Ramaprasad E. V. , Ramana C. V. . ( 2011; ). Rhodovulum phaeolacus sp. nov. a phototrophic alphaproteobacterium isolated from a brown pond. . J Gen Appl Microbiol 57:, 145–151. [CrossRef] [PubMed]
    [Google Scholar]
  16. Lane D. J. , Pace B. , Olsen G. J. , Stahl D. A. , Sogin M. L. , Pace N. R. . ( 1985; ). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. . Proc Natl Acad Sci U S A 82:, 6955–6959. [CrossRef] [PubMed]
    [Google Scholar]
  17. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  18. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Oren A. , Duker S. , Ritter S. . ( 1996; ). The polar lipid composition of Walsby’s square bacterium. . FEMS Microbiol Lett 138:, 135–140. [CrossRef]
    [Google Scholar]
  20. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Soto C. Y. , Cama M. , Gibert I. , Luquin M. . ( 2000; ). Application of an easy and reliable method for sulfolipid-I detection in the study of its distribution in Mycobacterium tuberculosis strains. . FEMS Microbiol Lett 187:, 103–107. [CrossRef] [PubMed]
    [Google Scholar]
  22. Srinivas T. N. R. , Anil Kumar P. , Sasikala Ch. , Spröer C. , Ramana ChV. . ( 2008; ). Rhodobacter ovatus sp. nov., a phototrophic alphaproteobacterium isolated from a polluted pond. . Int J Syst Evol Microbiol 58:, 1379–1383. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tindall B. J. . ( 1990a; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  25. Tindall B. J. . ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  26. Tindall B. J. , Rosselló-Móra R. , Busse H.-J. , Ludwig W. , Kämpfer P. . ( 2010; ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef] [PubMed]
    [Google Scholar]
  27. Trüper H. G. , Pfennig N. . ( 1981; ). Isolation of members of the families Chromatiaceae and Chlorobiaceae . . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279–289. Edited by Starr M. P. , Stolp H. , Trüper H. G. , Balows A. , Schlegel H. G. . . Berlin:: Springer;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.036152-0
Loading
/content/journal/ijsem/10.1099/ijs.0.036152-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error