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Abstract
A Gram-negative, non-spore-forming rod (CC-LN1-12T) was isolated from coastal soil samples of Lutao Island (Green Island), Taiwan, and its taxonomic position was studied. 16S rRNA gene sequence analysis showed that isolate CC-LN1-12T was grouped into the Microbulbifer cluster, with the highest similarities to Microbulbifer okinawensis ABABA23T (97.9 %), Microbulbifer maritimus TF-17T (97.7 %) and Microbulbifer donghaiensis CN85T (97.7 %), similarities to all other species of the genus Microbulbifer were lower than 96.8 %. The polyamine pattern contained the major compounds spermidine and cadaverine. The fatty acid profile, comprising the major fatty acids iso-C15 : 0, iso-C17 : 1ω9c, C18 : 1ω7c and iso-C11 : 0 3-OH as the major hydroxylated fatty acid, supported the affiliation of strain CC-LN1-12T to the genus Microbulbifer . DNA–DNA hybridizations between strain CC-LN1-12T and Microbulbifer okinawensis ABABA23T, M. donghaiensis CN85T and M. maritimus JCM 12187T resulted in relatedness values of 21.5 % (14.3 %, reciprocal analysis), 35.9 % (48.5 %, reciprocal analysis) and 48.1 % (52.1 %, reciprocal analysis), respectively. From these data, as well as from physiological and biochemical tests, strain CC-LN1-12T could be clearly differentiated from the most closely related species of the genus Microbulbifer . It is concluded that strain CC-LN1-12T represents a novel species, for which the name Microbulbifer taiwanensis sp. nov. is proposed. The type strain is CC-LN1-12T ( = LMG 26125T = CCM 7856T).
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