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Abstract

A bacterial strain designated antisso-13 was isolated from a salt pan in southern Taiwan and characterized using a polyphasic taxonomic approach. Strain antisso-13 was Gram-negative, aerobic, creamy white in colour, rod-shaped and motile by single monopolar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain antisso-13 belonged to the genus and its closest neighbour was MED92, with 96.7 % sequence similarity. Phylogenetic analyses based on and gene sequences and deduced amino acid sequences also revealed that the novel strain and MED92 formed a distinct phylogenetic cluster. Strain antisso-13 exhibited optimal growth at 25–30 °C, with 2.0–4.0 % NaCl and at pH 7.0. Cellular fatty acids were Cω7 (39.8 %), Cω7 (29.4 %), C (15.5 %), C 3-OH (7.5 %), anteiso-C (1.8 %), C (1.8 %), C 2-OH (1.6 %), iso-C (1.2 %) and C (1.1 %). The major respiratory quinone was ubiquinone Q-8. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine and several uncharacterized polar lipids and the DNA G+C content was 54.2 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from previously described members of the genus . It is evident from the genotypic and phenotypic data that strain antisso-13 should be classified as a representative of a novel species in the genus . The name proposed for this taxon is sp. nov.; the type strain is antisso-13 ( = LMG 25378 = BCRC 80079). An emended description of the genus is provided.

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2012-05-01
2021-05-19
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