%0 Journal Article %A Chen, Ming-Hui %A Sheu, Shih-Yi %A Chiu, Tzu-Fang %A Chen, Wen-Ming %T Neptuniibacter halophilus sp. nov., isolated from a salt pan, and emended description of the genus Neptuniibacter %D 2012 %J International Journal of Systematic and Evolutionary Microbiology, %V 62 %N Pt_5 %P 1104-1109 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.030379-0 %I Microbiology Society, %X A bacterial strain designated antisso-13T was isolated from a salt pan in southern Taiwan and characterized using a polyphasic taxonomic approach. Strain antisso-13T was Gram-negative, aerobic, creamy white in colour, rod-shaped and motile by single monopolar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain antisso-13T belonged to the genus Neptuniibacter and its closest neighbour was Neptuniibacter caesariensis MED92T, with 96.7 % sequence similarity. Phylogenetic analyses based on rpoB and recA gene sequences and deduced amino acid sequences also revealed that the novel strain and N. caesariensis MED92T formed a distinct phylogenetic cluster. Strain antisso-13T exhibited optimal growth at 25–30 °C, with 2.0–4.0 % NaCl and at pH 7.0. Cellular fatty acids were C16 : 1ω7c (39.8 %), C18 : 1ω7c (29.4 %), C16 : 0 (15.5 %), C10 : 0 3-OH (7.5 %), anteiso-C11 : 0 (1.8 %), C18 : 0 (1.8 %), C11 : 0 2-OH (1.6 %), iso-C10 : 0 (1.2 %) and C14 : 0 (1.1 %). The major respiratory quinone was ubiquinone Q-8. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine and several uncharacterized polar lipids and the DNA G+C content was 54.2 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from previously described members of the genus Neptuniibacter . It is evident from the genotypic and phenotypic data that strain antisso-13T should be classified as a representative of a novel species in the genus Neptuniibacter . The name proposed for this taxon is Neptuniibacter halophilus sp. nov.; the type strain is antisso-13T ( = LMG 25378T = BCRC 80079T). An emended description of the genus Neptuniibacter is provided. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.030379-0