1887

Abstract

A novel endophytic actinomycete, strain YIM 65646, was isolated from the roots of L. collected from Yunnan Province, south-west China. Growth was observed in the temperature range 10–40 °C (optimum 20–28 °C) and at pH 6.0–9.0 (optimum pH 7.0). The organism formed well-developed, branched substrate mycelia, but aerial mycelium was not produced. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain YIM 65646 belonged to the family (sharing ≤93.6 % sequence similarity with members of this family) and formed a distinct clade in the phylogenetic tree. The strain contained -diaminopimelic acid in the cell wall and mannose, ribose, galactose and glucose in whole-cell hydrolysates. The major menaquinones were MK-10(H), MK-10(H), MK-8(H), MK-9(H) and MK-10(H). The major fatty acids were iso-C, anteiso-C, anteiso-C, iso-C and iso-C. The DNA G+C content of strain YIM 65646 was 70.0 mol%. On the basis of morphological and chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain should be classified in a novel genus and species, gen. nov., sp. nov., in the family . The type strain is YIM 65646 ( = CCTCC AA 209041  = DSM 45386).

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2011-12-01
2019-10-18
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Scanning electron micrograph of strain YIM 65646 grown on ISP 2 medium for 30 days at 28 °C. Bar, 5 µm.

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Two-dimensional thin-layer chromatogram of polar lipids of strain YIM 65646 . The chromatographic conditions were as follows. Silica Gel 60 thin-layer plates (10 by 10 cm) were spotted with 15 µl of a whole-cell lipid extract. Chloroform/methanol/water (65 : 25 : 4, by vol.) was used in the first direction, and chloroform/acetic acid/methanol/water (80 : 18 : 12 : 5, by vol.) was used in the second direction. (a) Plate sprayed with α-naphthol reagent. (b) Plate sprayed with α-naphthol reagent and then with molybdenum blue reagent. (c) Polar lipid composition of strain YIM 65646 . A, Unknown glycolipid that stained red when the plate was sprayed with α-naphthol reagent (a) but did not change to blue with molybdenum blue reagent (b); 1, diphosphatidylglycerol; 2, phosphatidylglycerol; 3, phosphatidylcholine; 4, phosphatidylinositol; 5, phosphatidylmethylethanolamine; 6, phosphatidylethanolamine; 7, unknown glycolipid.

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