%0 Journal Article %A Li, Jie %A Zhao, Guo-Zhen %A Zhu, Wen-Yong %A Huang, Hai-Yu %A Xu, Li-Hua %A Zhang, Si %A Li, Wen-Jun %T Phytomonospora endophytica gen. nov., sp. nov., isolated from the roots of Artemisia annua L. %D 2011 %J International Journal of Systematic and Evolutionary Microbiology, %V 61 %N 12 %P 2967-2973 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.030205-0 %I Microbiology Society, %X A novel endophytic actinomycete, strain YIM 65646T, was isolated from the roots of Artemisia annua L. collected from Yunnan Province, south-west China. Growth was observed in the temperature range 10–40 °C (optimum 20–28 °C) and at pH 6.0–9.0 (optimum pH 7.0). The organism formed well-developed, branched substrate mycelia, but aerial mycelium was not produced. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain YIM 65646T belonged to the family Micromonosporaceae (sharing ≤93.6 % sequence similarity with members of this family) and formed a distinct clade in the Micromonosporaceae phylogenetic tree. The strain contained meso-diaminopimelic acid in the cell wall and mannose, ribose, galactose and glucose in whole-cell hydrolysates. The major menaquinones were MK-10(H4), MK-10(H2), MK-8(H2), MK-9(H2) and MK-10(H6). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0 and iso-C16 : 0. The DNA G+C content of strain YIM 65646T was 70.0 mol%. On the basis of morphological and chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain should be classified in a novel genus and species, Phytomonospora endophytica gen. nov., sp. nov., in the family Micromonosporaceae. The type strain is YIM 65646T ( = CCTCC AA 209041T  = DSM 45386T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.030205-0