1887

Abstract

Strain H2-LR, a 5–18 μm long and 0·7 μm wide filamentous, mesophilic, moderately halophilic, non-motile hydrogenotrophic methanogen, was isolated from marine sediment of Aarhus Bay, Denmark, 1·7 m below the sediment surface. On the basis of 16S rRNA gene comparison with sequences of known methanogens, strain H2-LR could be affiliated to the genus . The strain forms a distinct line of descent within this genus, with (95·9 % sequence identity) and (95·7 % sequence identity) as its closest relatives. The 16S rRNA-based affiliation was supported by comparison of the gene, which encodes the α-subunit of methyl-coenzyme M reductase. Strain H2-LR grew only on H/CO. The DNA G+C content is 34·9 mol%. Optimum growth temperature was 45 °C. The strain grew equally well at pH 7·5 and 8. No growth or methane production was observed below pH 5 or above pH 9. Strain H2-LR grew well within an NaCl concentration range of 100 and 900 mM. No growth or methane production was observed at 1 M NaCl. At 50 mM NaCl, growth and methane production were reduced. Based on 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel taxon within the genus , namely sp. nov. The type strain is H2-LR (=DSM 15219=ATCC BAA-828).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02994-0
2004-05-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540759.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02994-0&mimeType=html&fmt=ahah

References

  1. Boone, D. R. ( 2001; ). Genus I. Methanobacterium. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 215–218. Edited by D. R. Boone & R. W. Castenholz. New York: Springer-Verlag.
  2. Bryant, M. P. & Boone, D. R. ( 1987; ). Isolation and characterization of Methanobacterium formicicum MF. Int J Syst Bacteriol 37, 171.[CrossRef]
    [Google Scholar]
  3. Bryant, M. P., Wolin, E. A., Wolin, M. J. & Wolf, R. S. ( 1967; ). Methanobacillus omelianski, a symbiotic association of two species of bacteria. Arch Mikrobiol 59, 20–31.[CrossRef]
    [Google Scholar]
  4. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  5. Cuzin, N., Ouattara, A. S., Labat, M. & Garcia, J.-L. ( 2001; ). Methanobacterium congolense sp. nov., from a methanogenic fermentation of cassava peel. Int J Syst Evol Microbiol 51, 489–493.
    [Google Scholar]
  6. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). In Methods for General and Molecular Bacteriology, p. 31. Washington, DC: American Society for Microbiology.
  7. Großkopf, R., Janssen, P. H. & Liesack, W. ( 1998; ). Diversity and structure of the methanogenic community in anoxic rice paddy soil microcosms as examined by cultivation and direct 16S rRNA gene sequence retrieval. Appl Environ Microbiol 64, 960–969.
    [Google Scholar]
  8. Hales, B. A., Edwards, C., Ritchie, D. A., Hall, G., Pickup, R. W. & Saunders, J. R. ( 1996; ). Isolation and identification of methanogen-specific DNA from blanket bog peat by PCR amplification and sequence analysis. Appl Environ Microbiol 62, 668–675.
    [Google Scholar]
  9. Henckel, T., Friedrich, M. & Conrad, R. ( 1999; ). Molecular analyses of the methane-oxidizing microbial community in rice field soil by targeting the genes of the 16S rRNA, particulate methane monooxygenase, and methanol dehydrogenase. Appl Environ Microbiol 65, 1980–1990.
    [Google Scholar]
  10. Jain, M. K., Thompson, T. E., Conway de Macario, E. & Zelkus, J. G. ( 1987; ). Speciation of Methanobacterium strain Ivanov as Methanobacterium ivanovii sp. nov. Syst Appl Microbiol 9, 77–82.[CrossRef]
    [Google Scholar]
  11. Joulian, C., Ollivier, B., Patel, B. K. C. & Roger, P. A. ( 1998; ). Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils. FEMS Microbiol Ecol 25, 135–145.[CrossRef]
    [Google Scholar]
  12. Joulian, C., Patel, B. K. C., Ollivier, B., Garcia, J. L. & Roger, P. A. ( 2000; ). Methanobacterium orzyae sp. nov., a novel methanogenic rod isolated from a Philippines ricefield. Int J Syst Evol Microbiol 50, 525–528.[CrossRef]
    [Google Scholar]
  13. König, H. ( 1984; ). Isolation and characterization of Methanobacterium uliginosum sp. nov. from a marshy soil. Can J Microbiol 30, 1477–1481.[CrossRef]
    [Google Scholar]
  14. König, H. ( 1988; ). Archaeobacterial cell envelopes. Can J Microbiol 34, 395–406.[CrossRef]
    [Google Scholar]
  15. Kotelnikova, S., Macario, A. J. L. & Pedersen, K. ( 1998; ). Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen from deep granitic groundwater. Int J Syst Bacteriol 48, 357–367.[CrossRef]
    [Google Scholar]
  16. Lueders, T., Chin, K.-J., Conrad, R. & Friedrich, M. ( 2001; ). Molecular analyses of methyl-coenzyme M reductase alpha-subunit (mcrA) genes in rice field soil and enrichment cultures reveal the methanogenic phenotype of a novel archaeal lineage. Environ Microbiol 3, 194–204.[CrossRef]
    [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  18. Patel, G. B., Sprott, G. D. & Fein, J. E. ( 1990; ). Isolation and characterization of Methanobacterium espanolae sp. nov., a mesophilic moderately acidiphilic methanogen. Int J Syst Bacteriol 40, 12–18.[CrossRef]
    [Google Scholar]
  19. Pfennig, N. ( 1978; ). Rhodocyclus purpureus gen. nov. and sp. nov. a ring-shaped vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28, 283–288.[CrossRef]
    [Google Scholar]
  20. Simankova, M. V., Kotsyurbenko, O. R., Lueders, T., Nozhevnikova, A. N., Wagner, B., Conrad, R. & Friedrich, M. W. ( 2003; ). Isolation and characterization of new strains of methanogens from cold terrestrial habitats. Syst Appl Microbiol 26, 312–8.[CrossRef]
    [Google Scholar]
  21. Strunk, O. & Ludwig, W. ( 2000; ). ARB Software Package version 2.5b, Technische Universität, München, Germany. http://www.arb-home.de/
  22. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  23. Thomsen, T. R., Finster, K. & Ramsing, N. B. ( 2001; ). Biogeochemical and molecular signatures of anaerobic methane oxidation in a marine sediment. Appl Environ Microbiol 67, 1646–1656.[CrossRef]
    [Google Scholar]
  24. Visuvanathan, S., Moss, M. T., Stanford, J. L., Hermon-Taylor, J. & McFadden, J. J. ( 1989; ). Simple enzymatic method for isolation of DNA from diverse bacteria. J Microbiol Methods 10, 59–64.[CrossRef]
    [Google Scholar]
  25. Wasserfallen, A., Nolling, J., Pfister, P., Reeve, J. & Conway de Macario, E. ( 2000; ). Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supported the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermoautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov. Int J Syst Evol Microbiol 50, 43–53.[CrossRef]
    [Google Scholar]
  26. Whitman, W. B., Bowen, T. L. & Boone, D. R. ( 1992; ). The methanogenic bacteria. In The Prokaryotes, 2nd edn, vol. 1, p. 719–767. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer-Verlag.
  27. Widdel, F. & Bak, F. ( 1992; ). Gram-negative mesophilic sulphate-reducing bacteria. In The Prokaryotes, 2nd edn, vol. 4, pp. 3352–3378. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder, & K.-H. Schleifer. New York: Springer-Verlag.
  28. Zellner, G., Bleicher, K., Braun, E., Kneifel, H., Tindall, B. J., Conway de Macario, E. & Winter, J. ( 1989; ). Characterization of a new mesophilic, secondary alcohol-utilizing methanogen, Methanobacterium palustre spec. nov. from a peat bog. Arch Microbiol 151, 1–9.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02994-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02994-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error