%0 Journal Article %A Shlimon, Adris Georgis %A Friedrich, Michael W. %A Niemann, Helge %A Ramsing, Niels Birger %A Finster, Kai %T Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark) %D 2004 %J International Journal of Systematic and Evolutionary Microbiology, %V 54 %N 3 %P 759-763 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.02994-0 %I Microbiology Society, %X Strain H2-LRT, a 5–18 μm long and 0·7 μm wide filamentous, mesophilic, moderately halophilic, non-motile hydrogenotrophic methanogen, was isolated from marine sediment of Aarhus Bay, Denmark, 1·7 m below the sediment surface. On the basis of 16S rRNA gene comparison with sequences of known methanogens, strain H2-LRT could be affiliated to the genus Methanobacterium. The strain forms a distinct line of descent within this genus, with Methanobacterium oryzae (95·9 % sequence identity) and Methanobacterium bryantii (95·7 % sequence identity) as its closest relatives. The 16S rRNA-based affiliation was supported by comparison of the mcrA gene, which encodes the α-subunit of methyl-coenzyme M reductase. Strain H2-LRT grew only on H2/CO2. The DNA G+C content is 34·9 mol%. Optimum growth temperature was 45 °C. The strain grew equally well at pH 7·5 and 8. No growth or methane production was observed below pH 5 or above pH 9. Strain H2-LRT grew well within an NaCl concentration range of 100 and 900 mM. No growth or methane production was observed at 1 M NaCl. At 50 mM NaCl, growth and methane production were reduced. Based on 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel taxon within the genus Methanobacterium, namely Methanobacterium aarhusense sp. nov. The type strain is H2-LRT (=DSM 15219T=ATCC BAA-828T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02994-0