1887

Abstract

Gram-positive, spore-forming rods were isolated from blood cultures of three different patients. Based on phylogenetic analyses, these strains were placed within the cluster and specific phenotypic characteristics for each strain were described. Levels of 16S rRNA gene sequence similarity between existing species and the three novel strains 2301065, 2301032 and 2301083 were 87·6–94·4, 88·5–95·4 and 87·5–96·0 %, respectively, and anteiso-branched C was the major fatty acid. On the basis of phenotypic data and phylogenetic inference, it is proposed that these strains should be designated sp. nov., sp. nov. and sp. nov. The type strains are respectively strain 2301065 (=CIP 107939=CCUG 48215), strain 2301083 (=CIP 107938=CCUG 48214) and strain 2301032 (=CIP 108005=CCUG 48216).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02954-0
2004-07-01
2019-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/4/ijs541049.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02954-0&mimeType=html&fmt=ahah

References

  1. Aguilera, M., Monteoliva-Sanchez, M., Suarez, A., Guerra, V., Lizama, C., Bennasar, A. & Ramos-Cormenzana, A. ( 2001; ). Paenibacillus jamilae sp. nov., an exopolysaccharide-producing bacterium able to grow in olive-mill wastewater. Int J Syst Evol Microbiol 51, 1687–1692.[CrossRef]
    [Google Scholar]
  2. Antonello, A. & Weinstein, G. W. ( 1989; ). Successful treatment of Bacillus alvei endophthalmis. Am J Ophthalmol 108, 454–455.[CrossRef]
    [Google Scholar]
  3. Ash, C., Priest, F. G. & Collins, D. C. ( 1993; ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposals for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64, 253–260.
    [Google Scholar]
  4. Barrero, F., Galan, F., Marin, P., Garcia-Martos, P. & Capote, F. J. ( 1996; ). Catheter associated infection by Bacillus macerans in a patient with acute leukemia. Enferm Infect Microbiol Clin 14, 628–629.
    [Google Scholar]
  5. Berger, S. A. ( 1983; ). Pseudobacteremia due to contaminated alcohol swabs. J Clin Microbiol 18, 974–975.
    [Google Scholar]
  6. Bert, F., Ouahes, O. & Lambert-Zechovsky, N. ( 1995; ). Brain abscess due to Bacillus macerans following a penetrating periorbital injury. J Clin Microbiol 33, 1950–1953.
    [Google Scholar]
  7. Centers for Disease Control & Prevention (CDC) ( 1974; ). Recall of contaminated intravenous cannulae. Morb Mortal Wkly Rep 23, 57–58.
    [Google Scholar]
  8. Dasman, Kajiyama, S., Kawasaki, H., Yagi, M., Seki, T., Fukasaki, E. & Kobayashi, A. ( 2002; ). Paenibacillus glycanilyticus sp. nov., a novel species that degrades heteropolysaccharide produced by the corynebacterium Nostoc commune. Int J Syst Evol Microbiol 52, 1669–1674.[CrossRef]
    [Google Scholar]
  9. Elo, S., Suominen, I., Kämpfer, P., Juhanoja, J., Salkinoja-Salonen, M. & Haahtela, K. ( 2001; ). Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland. Int J Syst Evol Microbiol 51, 535–545.
    [Google Scholar]
  10. Heyndrickx, M., Vandemeulebroecke, K., Scheldeman, P., Kersters, K., De Vos, P., Logan, N. A., Aziz, A. M., Ali, N. & Berkeley, R. C. W. ( 1996; ). A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae. Int J Syst Bacteriol 46, 988–1003.[CrossRef]
    [Google Scholar]
  11. Ivanova, N., Sorokin, A., Anderson, I. & 20 other authors ( 2003; ). Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis. Nature 423, 87–91.[CrossRef]
    [Google Scholar]
  12. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  13. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetic analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  14. Kunst, F., Ogasawara, N., Moszer, I. & 148 other authors ( 1997; ). The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390, 249–256.[CrossRef]
    [Google Scholar]
  15. Meehan, C., Bjourson, A. J. & McMullan, G. ( 2001; ). Paenibacillus azotreducens sp. nov., a synthetic azo dye decolorizing bacterium from industrial wastewater. Int J Syst Evol Microbiol 51, 1681–1685.[CrossRef]
    [Google Scholar]
  16. Noskin, G. A., Suriano, T., Collins, S., Sesler, S. & Peterson, L. R. ( 2001; ). Clinical case study. Paenibacillus macerans pseudobacteremia resulting from contaminated blood culture bottles in a neonatal intensive care unit. Am J Infect Control 29, 126–129.[CrossRef]
    [Google Scholar]
  17. Read, T. D., Peterson, S. N., Tourasse, N. & 49 other authors ( 2003; ). The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 423, 81–86.[CrossRef]
    [Google Scholar]
  18. Reboli, A. C., Bryan, C. S. & Farrar, W. E. ( 1989; ). Bacteremia and infection of a hip prosthesis caused by Bacillus alvei. J Clin Microbiol 27, 1395–1396.
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997; ). Emended description of Paenibacillus amycolatus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis sp. nov. Int J Syst Bacteriol 47, 299–306.[CrossRef]
    [Google Scholar]
  21. Takeda, M., Kamagata, Y., Shinmaru, S., Nishiyama, T. & Koizumi, J. I. ( 2002; ). Paenibacillus koleovorans sp. nov., able to grow on the sheath of Sphaerotilus natans. Int J Syst Evol Microbiol 52, 1597–1601.[CrossRef]
    [Google Scholar]
  22. Teng, J. L. L., Woo, P. C. Y., Leung, K. W., Lau, S. K. P., Wong, M. K. M. & Yuen, K. Y. ( 2003; ). Pseudobacteremia in a patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov. J Clin Pathol 56, 29–35.
    [Google Scholar]
  23. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  24. Woese, C. R. ( 1987; ). Bacterial evolution. Microbiol Rev 51, 221–271.
    [Google Scholar]
  25. Yoon, J.-H., Oh, H.-M., Yoon, B.-D., Kang, K. H. & Park, Y.-H. ( 2003; ). Paenibacillus kribbensis sp. nov. and Paenibacillus terrae sp. nov., bioflocculants for efficient harvesting of algal cells. Int J Syst Evol Microbiol 53, 295–301.[CrossRef]
    [Google Scholar]
  26. York, M. K. ( 1990; ). Bacillus species pseudobacteremia traced to contaminated gloves used in collection of blood from patients with acquired immunodeficiency syndrome. J Clin Microbiol 28, 2114–2116.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02954-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02954-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error