1887

Abstract

A novel moderately thermophilic bacterium, strain TH13, was isolated from a hot spring in Kamchatka. It was found to be a Gram-positive, facultative aerobe; the straight, non-motile rods grew at 30–64 °C (optimum 54 °C). The isolate was positive for catalase and oxidase tests and reduced nitrate to nitrite, but was negative for HS production and growth in more than 3 % NaCl (w/v). The isolate grew at pH 7–8, but not at pH values higher than 9. The DNA G+C content was 43·9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain TH13 was a member of the genus . DNA–DNA hybridization revealed a low relatedness (less than 30·2 %) between the isolate and its close phylogenetic neighbours and . On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA hybridization data, it was concluded that the isolate merited classification as a novel species, for which the name sp. nov. is proposed. The type strain of this species is TH13 (=NCIMB 13956=JCM 12111).

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2004-07-01
2021-04-23
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References

  1. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Belduz A. O., Dulger S., Demirbag Z. 2003; Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 53:1315–1320 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Hanada S., Hiraishi A., Shimada K., Matsuura K. 1995; Chloroflexus aggregans sp. nov., a filamentous phototrophic bacterium which forms dense cell aggregates by active gliding movement. Int J Syst Bacteriol 45:676–681 [CrossRef]
    [Google Scholar]
  5. Hanada S., Takaichi S., Matsuura K., Nakamura K. 2002; Roseiflexus castenholzii gen. nov., sp. nov. a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52:187–193
    [Google Scholar]
  6. Heinen W., Lauwers A. M., Mulders J. W. M. 1982; Bacillus flavothermus , a newly isolated facultative thermophile. Antonie van Leeuwenhoek 48:265–272 [CrossRef]
    [Google Scholar]
  7. Hiraishi A., Umezawa T., Yamamoto H., Kato K., Yonosuke M. 1999; Changes in quinone profiles of hot spring microbial mats with a thermal gradient. Appl Environ Microbiol 65:198–205
    [Google Scholar]
  8. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  9. Kawasaki K., Nogi Y., Hishinuma M., Nodasaka Y., Matsuyama H., Yumoto I. 2002; Psychromonas marina sp. nov., a novel halophilic, facultatively psychrophilic bacterium isolated from the coast of the Okhotsk Sea. Int J Syst Evol Microbiol 52:1455–1459 [CrossRef]
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  12. Nübel U., Bateson M. M., Vandieken V., Wieland A., Kühl M., Ward D. M. 2002; Microscopic examination of distribution and phenotypic properties of phylogenetically diverse Chloroflexaceae -related bacteria in hot spring microbial mat. Appl Environ Microbiol 68:4593–4603 [CrossRef]
    [Google Scholar]
  13. Pierson B. K., Giovannoni S. J., Stahl D. A., Castenholz R. W. 1985; Heliothrix oregonensis , gen. nov., a phototrophic filamentous gliding bacterium containing bacteriochlorophyll a . Arch Microbiol 142:164–167 [CrossRef]
    [Google Scholar]
  14. Pikuta E., Lysenko A., Chuvilskaya N., Mendrock U., Hippe H., Suzina N., Nikitin D., Osipov G., Laurinavichius K. 2000; Anoxybacillus pushchinensis gen. nov., sp. nov. a novel anaerobic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov. Int J Syst Evol Microbiol 50:2109–2117 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Yamamoto H., Hiraishi A., Kato K., Chiura H. X., Maki Y., Shimizu A. 1998; Phylogenetic evidence for the existence of novel thermophilic bacteria in hot spring sulfur-turf microbial mats in Japan. Appl Environ Microbiol 64:1680–1687
    [Google Scholar]
  19. Yumoto I., Yamazaki K., Hishinuma M., Nodasaka Y., Inoue N., Kawasaki K. 2000; Identification of facultatively alkaliphilic Bacillus sp. strain YN-2000 and its fatty acid composition and cell-surface aspects depending on culture pH. Extremophiles 4:285–290 [CrossRef]
    [Google Scholar]
  20. Yumoto I., Yamazaki K., Hishinuma M., Nodasaka Y., Suemori A., Nakajima K., Inoue N., Kawasaki K. 2001; Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. Int J Syst Evol Microbiol 51:349–355
    [Google Scholar]
  21. Yumoto I., Nakamura A., Iwata H., Kojima K., Kusumoto K., Nodasaka Y., Matsuyama H. 2002; Dietzia psychralcaliphila sp. nov., a novel, facultatively psychrophilic alkaliphile that grows on hydrocarbons. Int J Syst Evol Microbiol 52:85–90
    [Google Scholar]
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