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Abstract

A novel moderately thermophilic bacterium, strain TH13, was isolated from a hot spring in Kamchatka. It was found to be a Gram-positive, facultative aerobe; the straight, non-motile rods grew at 30–64 °C (optimum 54 °C). The isolate was positive for catalase and oxidase tests and reduced nitrate to nitrite, but was negative for HS production and growth in more than 3 % NaCl (w/v). The isolate grew at pH 7–8, but not at pH values higher than 9. The DNA G+C content was 43·9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain TH13 was a member of the genus . DNA–DNA hybridization revealed a low relatedness (less than 30·2 %) between the isolate and its close phylogenetic neighbours and . On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA hybridization data, it was concluded that the isolate merited classification as a novel species, for which the name sp. nov. is proposed. The type strain of this species is TH13 (=NCIMB 13956=JCM 12111).

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2004-07-01
2019-10-16
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vol. , part 4, pp. 1239-1242

Phylogenetic tree constructed based on 16S rRNA gene sequence data of TH13 and other related organisms using the neighbour-joining method. Bootstrap analysis shows the robustness of the branching. Bar, 0.01 unit.



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