1887

Abstract

Five strains with strong chitinolytic activity were isolated from a soil sample collected from southern Taiwan. The strains shared more than 92 % DNA–DNA similarity, indicating membership of the same genospecies. This close relationship was supported by high similarities in fatty acid composition and biochemical characteristics. A 16S rRNA gene sequence analysis indicated that the isolates were members of the class ‘’, in which they formed an individual subline of descent that was distantly related (<94 % similarity) to lineages defined by DSM 6150 and DSM 3764. On the basis of the phylogenetic and phenotypic distinctness of these novel chitin-degrading organisms, a new genus, , is proposed, with (type strain, S1=BCRC 17254=DSM 15459) as the type species.

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2004-07-01
2019-10-20
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References

  1. Bakkers, J., Semino, C. E., Stroband, H., Kijne, J. W., Robbins, P. W. & Spaink, H. P. ( 1997; ). An important developmental role for oligosaccharides during early embryogenesis of cyprinid fish. Proc Natl Acad Sci U S A 94, 7982–7986.[CrossRef]
    [Google Scholar]
  2. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  3. Cappuccino, J. G. & Sherman, N. ( 1987; ). Microbiology: a Laboratory Manual. Menlo Park, CA: Benjamin/Cummings Publishing.
  4. DSMZ ( 2001; ). Catalogue of Strains, 7th edn. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zelkuturen.
  5. Collins, M. D. & Jones, D. ( 1981; ). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51, 129–134.[CrossRef]
    [Google Scholar]
  6. DeSoete, G. ( 1983; ). A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef]
    [Google Scholar]
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  9. Flach, J., Pilet, P. E. & Jolles, P. ( 1992; ). What's new in chitinase research? Experientia 48, 701–716.[CrossRef]
    [Google Scholar]
  10. Franke, I. H., Fegan, M., Hayward, C., Leonard, G., Stackebrandt, E. & Sly, L. I. ( 1999; ). Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug. Int J Syst Bacteriol 49, 1681–1693.[CrossRef]
    [Google Scholar]
  11. Hippe, H., Hagelstein, A., Kramer, I., Swiderski, J. & Stackebrandt, E. ( 1999; ). Phylogenetic analysis of Formivibrio citricus, Propionivibrio dicarboxylicus, Anaerobiospirillum thomasii, Succinimonas amylolytica and Succinivibrio dextrinosolvens and proposal of Succinivibrionaceae fam. nov. Int J Syst Bacteriol 49, 779–782.[CrossRef]
    [Google Scholar]
  12. Hsu, S. C. & Lockwood, J. L. ( 1975; ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29, 422–426.
    [Google Scholar]
  13. Imoto, T. & Yagishita, K. ( 1971; ). A simple activity measurement of lysozyme. Agric Biol Chem 35, 1154–1156.[CrossRef]
    [Google Scholar]
  14. Koneman, E., Allen, S., Dowell, V. & Sommers, H. ( 1979; ). Color Atlas and Textbook of Diagnostic Microbiology, pp. 216–217. Philadelphia: J. B. Lippincott.
  15. Logan, N. A. ( 1989; ). Numerical taxonomy of violet-pigmented, Gram-negative bacteria and description of Iodobacter fluviatile gen. nov., comb. nov. Int J Syst Bacteriol 39, 450–456.[CrossRef]
    [Google Scholar]
  16. Monreal, J. & Reese, E. T. ( 1969; ). The chitinase of Serratia marcescens. Can J Microbiol 15, 689–696.[CrossRef]
    [Google Scholar]
  17. Sampson, M. N. & Gooday, G. W. ( 1998; ). Involvement of chitinases of Bacillus thuringiensis during pathogenesis in insects. Microbiology 144, 2189–2194.[CrossRef]
    [Google Scholar]
  18. Suzuki, K., Mikami, T., Okawa, Y., Tokoro, A., Suzuki, S. & Suzuki, M. ( 1986; ). Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose. Carbohydr Res 151, 403–408.[CrossRef]
    [Google Scholar]
  19. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  20. Tanaka, K., Nakamura, K. & Mikami, E. ( 1991; ). Fermentation of S-citramalate, citrate, mesaconate, and pyruvate by a Gram-negative strictly anaerobic non-spore-former, Formivibrio citricus gen. nov., sp. nov. Arch Microbiol 155, 491–495.[CrossRef]
    [Google Scholar]
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vol. , part 4, pp. 1387–1391

The phenotypic characteristics (Table A) and cellular fatty acid compositions (Table B) of the novel chitin-degrading strains, and DNA–DNA hybridization results (Table C), are available to download. [PDF](134KB)



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